Product nameAnti-Myc tag antibody
See all Myc tag primary antibodies
DescriptionRabbit polyclonal to Myc tag
SpecificityELISA: Antibody specificity was verified by ELISA against the peptide (EQKLISEEDL). A 1:60,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP-conjugated Goat Anti Rabbit IgG at 1:20,000 and TMB as the substrate. Appropriate specificity controls were run.
Tested applicationsSuitable for: IHC-Fr, IP, WB, IHC-P, Flow Cyt, ICC/IF, ICC, Electron Microscopymore details
- ICC/IF: HeLa cells transfected with a Myc-tagged membrane targeting protein. IP: Huh7 cells expressing myc-tagged CDC42 protein. Human 293FT cells co-transfected with myc tagged cdc25 along with an HA tagged viral protein which binds to cdc25.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
Purification notesAntibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg of IgG.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab9106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Can be blocked with Human c-Myc peptide (ab13837).|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 19793804
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration. PubMed: 16093346|
|ICC||Use at an assay dependent concentration.|
|Electron Microscopy||Use at an assay dependent concentration. See Abreview for further details (submitted by Eeva-Liisa Eskelinen).|
RelevanceEpitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
- c-myc tag antibody
- Myc Epitope Tag antibody
Y. enterocolitica ΔHOPEMT carrying plasmids encoding Myc-tagged CT082 or CT695, and HA-tagged Slc1 (as indicated) were grown in non-secreting conditions. Chloramphenicol was added (time?=?0 min) to stop bacterial protein synthesis and samples were taken at the indicated time points. Samples were analyzed by immunoblotting using ab9106, anti-HA, and anti-TEM antibodies.
ab9106 staining the Myc tag in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells transfected with a Myc-tagged membrane targeting protein by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde, permeabilized with Triton X (0.5%) and blocked with 3% BSA for 1 hour at 24°C. Samples were incubated with primary antibody (1/500) for 1 hour at 24°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
ab9106 at 4 µg/mg lysate used in Huh7 (Human cell line) whole cell lysate (1x106 cells).
Cells expressed myc-tagged CDC42 protein. Immunoprecipitation step performed using Protein A matrix. Incubation time 4 hours at 4°C. Western Blot antibody used at 1/300 dilution.
ab9106 used at 1/250 in the immunoprecipitation of transfected human 293FT cells (whole cell lysate).
In this experiment cells were co-transfected with myc tagged cdc25 along with an HA tagged viral protein which binds to cdc25. The cell lysate was immunoprecipitated using ab9106, run on a 10% gel. The blot was probed for HA and HA tagged protein (which co-IPed with Myc tagged protein) and this is seen at ~15KDa.
Lane 1: Input non-transfected cells
Lane 2: Immunoprecipitated non-transfected cells
Lane 3: Input co-transfected cells
Lane 4: Immunoprecipitated co-transfected cells
This image is courtesy of an Abreview submitted on 23 March 2006.
ab9106 at 1/2000 staining mouse embryonic fibroblasts expressing myc-tagged protein by ICC.
The cells were paraformaldehyde fixed and blocked with 3% BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit IgG antibody (10 nm gold conjugated) was used as the secondary.
The image shown is immuno electron microscopical staining with thawed cryosections.
This product has been referenced in:
- Tögel L et al. DUSP5 is methylated in CIMP-high colorectal cancer but is not a major regulator of intestinal cell proliferation and tumorigenesis. Sci Rep 8:1767 (2018). Read more (PubMed: 29379130) »
- Jong WSP et al. Comparing autotransporter ß-domain configurations for their capacity to secrete heterologous proteins to the cell surface. PLoS One 13:e0191622 (2018). Read more (PubMed: 29415042) »