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Here is the point-wise reply to your queries:
1) More than the delay, it's the improper temperature at which the Ab was
2) There was no band on the blot.
3) So we transfected our cells with our gene of interest with a myc tag. On
doing WB using Ab again our protein of interest we can clearly see the
expression both in WB and IF. So I don't think it has anything to do with
transfection. The primary reason for this Ab not to work is the way it reached
to the lab.
4) Unfortunately I didn't save the image. I have reason for that. In India we
have very limited budget and we try to conserve the reagents. Since I didn't see
the band, I decided not to wast the downstream reagent.
Looking forward towards your response.
Asked on Jan 08 2013
Thank you for your email.
Unfortunately the delay wasn't from Abcam, we have shipped the product at correct temperature. Please check your delivery service and let them know these products are temperature sensitive and they need to be delivered without delays.
We will be shipping a new vial as replacement to the next order of Nulife. Please make sure the customer gets this vial without delays. The new order number is 1229622.
I hope this information will be helpful. Should you have any question please do not hesitate to contact me.
Answered on Jan 08 2013