Immunocytochemistry abreview for Anti-Myc tag antibody

Excellent
Abreviews
Application
Immunocytochemistry
Sample
Human Cultured Cells (HELA)
Specification
HELA
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-x-0.5%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
Cells transfected with a Myc-tagged membrane targeting protein

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Submitted Oct 04 2010

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