Western blot abreview for Anti-Myc tag antibody

Poor
Abreviews
Application
Western blot
Sample
Tobacco Tissue lysate - whole (leaf)
Loading amount
20 µg
Specification
leaf
Gel Running Conditions
Non-reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Dilution
1/3000
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: ECL anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/8000

Detection

Detection method
ECL+
Exposure
15 second(s)
Positive control
FEA2 with myc
Negative control
non transfected

Additional data

Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

Abcam user community

Verified customer

Submitted Jan 28 2013

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