Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myc tag antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
0.3% BSA/8%donkeyserum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (brain)
Specification
brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3% BSA/0.33%TritonX-100/8%donkeyserum
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: alexa fluor donkey anti-rabbit 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 555
Dilution
1/500

Additional data

Additional Notes
This is a brain electroplated at E14.5 with EGFP and a myc tagged protein, and then perfused at P28.Tissue then fixed with 4% PFA overnight, embedded in agar and sectioned on vibratome.

Karen Yee

Verified customer

Submitted Oct 02 2014

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