Immunoprecipitation abreview for Anti-Myc tag antibody

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Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293 FT)
Total protein in input
500 µg
Specification
293 FT
Immuno-precipitation step
Protein A/G

Other product details

Dilution
1/250
Incubation time
4 hour(s) and 0 minute(s)

Western Blot antibody

Name
Non-Abcam antibody was used: goat anti mouse
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Lane 1 is input of untrasfected cells, lane 2 is IP using myc antibody of untransfected cells, lane 3 is input from cells co-transfected with a Myc-tagged cdc25 and an HA tagged viral protein known to bind cdc25, lane4 is IP with myc antibody of cells transfected with myc tagged cdc25 and HA tagged viral protein. The blot was Probed for HA and HA tagged protein which co-IPed with Myc tagged protein is at ~15KDa. 1mg of wcl was used for the IP and half of the IP was loaded on the gel. In addition 10ug of wcl was loaded for input control (lanes 1 and 3).

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Submitted Apr 25 2006

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