Non-Abcam antibody was used: goat anti mouse Host species: Goat Clonality: Monoclonal Conjugation: Horse Radish Peroxidase
Lane 1 is input of untrasfected cells, lane 2 is IP using myc antibody of untransfected cells, lane 3 is input from cells co-transfected with a Myc-tagged cdc25 and an HA tagged viral protein known to bind cdc25, lane4 is IP with myc antibody of cells transfected with myc tagged cdc25 and HA tagged viral protein. The blot was Probed for HA and HA tagged protein which co-IPed with Myc tagged protein is at ~15KDa. 1mg of wcl was used for the IP and half of the IP was loaded on the gel. In addition 10ug of wcl was loaded for input control (lanes 1 and 3).
Abcam user community
Submitted Apr 25 2006
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