Western blot abreview for Anti-Myc tag antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293 Cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
Transfected with Myc-GFP plasmid.
Specification
293 Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG, HRP Linked
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Negative control
Untransfected control cells.
Exposure
2 second(s)
Bands
Specific: 45 kDa Non-specific: 100 kDa
Positive control
Cells transfected with Myc-GFP plasmids.

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Submitted Aug 19 2015

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