Western blot abreview for Anti-Myc tag antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Treatment
Cells tranfected with empty vector or myc-tagged SIRT1 followed by IP SIRT1.
Specification
HEK293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name

Detection

Detection method
ECL
Negative control
Non-transfected cell lysate
Exposure
10 second(s)
Bands
Specific: ~116 kDa

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Submitted Dec 06 2017

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