Immunocytochemistry/ Immunofluorescence abreview for Anti-Myc tag antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human microglial cell line)
Permeabilization
Yes - 0.1% triton X-100
Specification
human microglial cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS/5% goat serum/0.1% Triton X-100
Dilution
1/500

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: goat anti-rabbit 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
Image shows human microglial cells transduced with a lentiviral vector containing a Myc-tagged lysosomal enzyme. Transduced cells showed Myc-reactivity.

Abcam user community

Verified customer

Submitted May 09 2018

Sign up