Western blot abreview for Anti-Myc tag antibody

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
3e+006 cells
Treatment
Transfected with 10µg of expression vector encoding a Myc-fused protein.
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Solution
Dilution
1/2000

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: NA934, Amersham ECL Rabbit IgG, HRP whole Ab
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 22 kDa Non-specific: 60, 75 kDa

Additional data

Additional Notes
The specif bands are clear, but there are also non-specific bands. Probably, our standard WB protocol needs to be optimised for this antibody.

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Submitted Jul 16 2018

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