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Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (7.5%)
Transfected with 10µg of expression vector encoding a Myc-fused protein.
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Solution
Non-Abcam antibody was used: NA934, Amersham ECL Rabbit IgG, HRP whole Ab
Host species: Donkey
Conjugation: Horse Radish Peroxidase
5 minute(s) and 0 second(s)
Specific: 22 kDa Non-specific: 60, 75 kDa
The specif bands are clear, but there are also non-specific bands. Probably, our standard WB protocol needs to be optimised for this antibody.
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Submitted Jul 16 2018