Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Light chain type
    unknown
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab9109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/200 - 1/500 (coating) and 1/5000 – 1/20000 (probing).
    WB: 1/5000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
    • Cellular localization
      Nuclear
    • Alternative names
      • c-myc tag antibody
      • Myc Epitope Tag antibody

    References

    This product has been referenced in:
    • Wang F  et al. Ca2+ homeostasis modulation enhances the amenability of L444P glucosylcerebrosidase to proteostasis regulation in patient-derived fibroblasts. ACS Chem Biol 6:158-68 (2011). ELISA . Read more (PubMed: 21043486) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you very much for contacting us with your question.

    Since the antibody ab9109 is specific for the Myc tag and not an endogenousDrosophila protein, we would guarantee this antibody to work in the tested applications of Western blot and ELISA with your samples. However, we have not tested ab9109 in any kind of immunostaining so we're not sure if it is suitable for this application. We don't provide sample aliquots of our products, but we do have a testing discount program that you are eligible for. As part of this program, you would need to first purchase ab9109 at full price, but after testing the antibody in immunostaining and submitting an online Abreview with your data and protocol, you would receive one free primary antibody on a future purchase.If you're interested in this program I can send you the full details.

    Wealso have several anti-chicken secondary antibodies in case you don't already have one. For an HRP conjugation, I would recommend ab7118, which has been tested in immunostaining and has been pre-adsorbed so that it won't cross-react with your other primary antibody-

    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Chicken-IgY-HL-HRP-pre-adsorbed-ab7118.html

    For fluorescent detection, I would suggest ab96951 DyLight 488 conjugated, which has also been tested in immunostaining and is pre-adsorbed-

    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Chicken-IgY-H-L-DyLight-488-pre-adsorbed-ab96951.html

    I hope that this information will be useful, but please let me know if you have any further questions or require further informaiton. I'll be happy to help. Have a nice day!

    Read More

    Answer

    We do not have any data on the use of this antibody in immunofluorescence. However, if you are testing the antibody in this application, please let us know your results and in return we'll forward a EUR 25.00 Amazon gift voucher.

    Read More

    Answer

    I'm sorry, I don't quite understand your question. In WB, this antibody is reported to be able to detect the c-myc epitope tag at a 1:10,000 dilution.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up