Key features and details
- Goat polyclonal to Myc tag - ChIP Grade
- Suitable for: IP, ChIP, IHC-Fr, ICC/IF, WB, Flow Cyt, ICC, ELISA
- Isotype: IgG
Product nameAnti-Myc tag antibody - ChIP Grade
See all Myc tag primary antibodies
DescriptionGoat polyclonal to Myc tag - ChIP Grade
SpecificityAntibody was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL /BSA).
Tested applicationsSuitable for: IP, ChIP, IHC-Fr, ICC/IF, WB, Flow Cyt, ICC, ELISAmore details
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
ChIP Related Products
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab9132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 1-4 µg/mg of lysate.
Also PubMed: 20224777
|ChIP||Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000 - 1/30000. Can be blocked with Human c-Myc peptide (ab13837).|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 22158898
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC||1/200 - 1/2000.|
|ELISA||1/100 - 1/500.|
RelevanceEpitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
- c-myc tag antibody
- Myc Epitope Tag antibody
All lanes : Anti-Myc tag antibody - ChIP Grade (ab9132) at 0.04 µg/ml
Lane 1 : E. coli whole cell lysate at 0.2 µg
Lane 2 : E. coli whole cell lysate at 0.1 µg
Lane 3 : E. coli whole cell lysate at 0.05 µg
A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
ab9132, at 1/500, tagging the Myc epitope in human Hela cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in 0.15% Triton prior to blocking in 10% serum for 1 hour at 23°C. The primary antibody incubated with the sample for 1 hour at 23°C. A donkey polyclonal anti-goat antibody, diluted 1/500, was used as the secondary.
ab9132, at 1/50 tagging Myc epitope in zebrafish frozen skeleton section by immunohistochemistry. Tissue was Paraformaldehyde fixed and blocked with 5% BSA for 4 hours at 25oC. The sample was incubated with the primary antibody for 16 hours at 4oC followed by the secondary, Rabbit Anti-Goat Cy3® at 1/500 dilution.
ab9132 has been referenced in 99 publications.
- Péladeau C et al. Identification of therapeutics that target eEF1A2 and upregulate utrophin A translation in dystrophic muscles. Nat Commun 11:1990 (2020). PubMed: 32332749
- Rubio K et al. Inactivation of nuclear histone deacetylases by EP300 disrupts the MiCEE complex in idiopathic pulmonary fibrosis. Nat Commun 10:2229 (2019). PubMed: 31110176
- Li H et al. Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations. Autophagy 15:113-130 (2019). PubMed: 30160596
- Pegg HJ et al. The RUNX Transcriptional Coregulator, CBFß, Suppresses Migration of ER+ Breast Cancer Cells by Repressing ERa-Mediated Expression of the Migratory Factor TFF1. Mol Cancer Res N/A:N/A (2019). PubMed: 30655324
- Yi DR et al. Human MxB Inhibits the Replication of Hepatitis C Virus. J Virol 93:N/A (2019). PubMed: 30333168