Overview

  • Product name
    Anti-Myc tag antibody - ChIP Grade
    See all Myc tag primary antibodies
  • Description
    Goat polyclonal to Myc tag - ChIP Grade
  • Host species
    Goat
  • Specificity
    Antibody was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL /BSA).
  • Tested applications
    Suitable for: IP, ChIP, IHC-Fr, ICC/IF, WB, Flow Cyt, ICC, ELISAmore details
  • Immunogen

    Synthetic peptide:

    EQKLISEEDL

    (c-myc) conjugated to KLH.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 1-4 µg/mg of lysate.

Also PubMed: 20224777 

 

ChIP Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.
IHC-Fr 1/50.
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/30000. Can be blocked with Human c-Myc peptide (ab13837).
Flow Cyt Use at an assay dependent concentration. PubMed: 22158898

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC 1/200 - 1/2000.
ELISA 1/100 - 1/500.

Target

  • Relevance
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization
    Nuclear
  • Alternative names
    • c-myc tag antibody
    • Myc Epitope Tag antibody

Images

  • All lanes : Anti-Myc tag antibody - ChIP Grade (ab9132) at 0.04 µg/ml

    Lane 1 : E. coli whole cell lysate at 0.2 µg
    Lane 2 : E. coli whole cell lysate at 0.1 µg
    Lane 3 : E. coli whole cell lysate at 0.05 µg
  • A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
  • ab9132, at 1/500, tagging the Myc epitope in human Hela cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in 0.15% Triton prior to blocking in 10% serum for 1 hour at 23°C. The primary antibody incubated with the sample for 1 hour at 23°C. A donkey polyclonal anti-goat antibody, diluted 1/500, was used as the secondary.

    See Abreview

  • ab9132, at 1/50 tagging Myc epitope in zebrafish frozen skeleton section by immunohistochemistry. Tissue was Paraformaldehyde fixed and blocked with 5% BSA for 4 hours at 25oC. The sample was incubated with the primary antibody for 16 hours at 4oC followed by the secondary, Rabbit Anti-Goat Cy3® at 1/500 dilution.  

    See Abreview

References

This product has been referenced in:
See all 82 Publications for this product

Customer reviews and Q&As

11-12 of 12 Abreviews or Q&A

Question
Answer

No, this antibody has not yet been tested in IF

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Question
Answer

Cross reactivity to n-myc has not been tested. The immunogen was the peptide sequence EQKLISEEDL.

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11-12 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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