Overview

  • Product name
    Anti-Myc tag antibody - ChIP Grade
    See all Myc tag primary antibodies
  • Description
    Goat polyclonal to Myc tag - ChIP Grade
  • Host species
    Goat
  • Specificity
    Antibody was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL /BSA).
  • Tested applications
    Suitable for: IP, ChIP, IHC-Fr, ICC/IF, WB, Flow Cyt, ICC, ELISAmore details
  • Immunogen

    Synthetic peptide:

    EQKLISEEDL

    (c-myc) conjugated to KLH.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 1-4 µg/mg of lysate.

Also PubMed: 20224777 

 

ChIP Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.
IHC-Fr 1/50.
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/30000. Can be blocked with Human c-Myc peptide (ab13837).
Flow Cyt Use at an assay dependent concentration. PubMed: 22158898

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC 1/200 - 1/2000.
ELISA 1/100 - 1/500.

Target

  • Relevance
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization
    Nuclear
  • Alternative names
    • c-myc tag antibody
    • Myc Epitope Tag antibody

Images

  • All lanes : Anti-Myc tag antibody - ChIP Grade (ab9132) at 0.04 µg/ml

    Lane 1 : E. coli whole cell lysate at 0.2 µg
    Lane 2 : E. coli whole cell lysate at 0.1 µg
    Lane 3 : E. coli whole cell lysate at 0.05 µg
  • A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
  • ab9132, at 1/500, tagging the Myc epitope in human Hela cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in 0.15% Triton prior to blocking in 10% serum for 1 hour at 23°C. The primary antibody incubated with the sample for 1 hour at 23°C. A donkey polyclonal anti-goat antibody, diluted 1/500, was used as the secondary.

    See Abreview

  • ab9132, at 1/50 tagging Myc epitope in zebrafish frozen skeleton section by immunohistochemistry. Tissue was Paraformaldehyde fixed and blocked with 5% BSA for 4 hours at 25oC. The sample was incubated with the primary antibody for 16 hours at 4oC followed by the secondary, Rabbit Anti-Goat Cy3® at 1/500 dilution.  

    See Abreview

References

This product has been referenced in:
  • Murakami T  et al. Cutting Edge: G Protein Subunit ß 1 Negatively Regulates NLRP3 Inflammasome Activation. J Immunol 202:1942-1947 (2019). Read more (PubMed: 30777924) »
  • Rapanelli M  et al. Striatal Signaling Regulated by the H3R Histamine Receptor in a Mouse Model of tic Pathophysiology. Neuroscience 392:172-179 (2018). Read more (PubMed: 30278251) »
See all 74 Publications for this product

Customer reviews and Q&As

1-6 of 6 Q&A

Answer

You can find out more about the 100% Abreview Testing Discount Program below:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



For all the antibodies that you are interested in, we already guarantee them to work in ChIP experiments, regardless of species. Therefore, they would not qualify for the testing discount program, but they are covered under our Abpromise.

If there is anything else I can help you with, or if you are interested in any of our other antibodies that have not been tested in ChIP, but you might be interested in them, then please let me know.

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Answer

Thank you for contacting Abcam. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Answer

Thank you very much for your call today and for your question about ab9132 and our other myc tag antibodies.

I couldn't find any data specifically showing that ab9132 has been tested for reactivity against endogenous c-myc, however since the myc tag sequence is homologous with the c-myc sequence, we do expect that these antibodies will cross-react with endogenous c-myc.

I am sorry that we do not have any more information at this time. If you have any further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

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Question
Answer

Thank you for your enquiry. Unfortunately the antibody has not been tested on endogenous mouse protein, so there is no data to say either way. I am sorry I could not be more helpful. Please do not hesitate to contact us if you need anything further.

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Question
Answer

No, this antibody has not yet been tested in IF

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Question
Answer

Cross reactivity to n-myc has not been tested. The immunogen was the peptide sequence EQKLISEEDL.

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