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Human Cell lysate - whole cell (293 cell)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Non-Abcam antibody was used: Donkey anti-Goat
Host species: Donkey
Conjugation: Horse Radish Peroxidase
Specific: 110 kDa Non-specific: None kDa
Nitrocellulose Membrane (Pierce). Negative Control is run in lane 1 with a a myc-sample run in the lane showing a positive result.
Please note that we used a Femto ECL system which allows us to use a much more diluted concentration of our anti-bodies.
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Submitted Aug 14 2009