Western blot abreview for Anti-Myc tag antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293 cell)
Loading amount
20 µg
Specification
293 cell
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Dilution
1/30000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-Goat
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/200000

Detection

Detection method
ECL (Femto-Piece)
Exposure
10 second(s)
Bands
Specific: 110 kDa Non-specific: None kDa

Additional data

Additional Notes
Nitrocellulose Membrane (Pierce). Negative Control is run in lane 1 with a a myc-sample run in the lane showing a positive result.

Please note that we used a Femto ECL system which allows us to use a much more diluted concentration of our anti-bodies.

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Verified customer

Submitted Aug 14 2009

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