ChIP abreview for Anti-Myc tag antibody - ChIP Grade

Good
Abreviews
Application
ChIP
Sample
Human Cell lysate - other (HEK cell Chromatin)
Specification
HEK cell Chromatin
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step
Real-time PCR
Positive control
Histone H3 antibody
Negative control
IGG antibody, EV Control

Other product details

Concentration
10 µg/µg chromatin
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Chip Kit Dilution buffer

Additional data

Additional Notes
I performed this Assay with HEK cells transfected with a myc-tagged Protein of Interest using a commercially available CHIP Kit. I had to increase the antibody concentration to 10 µg/10µg Chromatin. The Kit used IgG and Histone antibodies as controls, so I normalized to IgG control and had a significant increase in DNA in qPCR compared to EV-myc transfected cells.
POI = protein of interest.

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Submitted Oct 29 2010

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