I performed this Assay with HEK cells transfected with a myc-tagged Protein of Interest using a commercially available CHIP Kit. I had to increase the antibody concentration to 10 µg/10µg Chromatin. The Kit used IgG and Histone antibodies as controls, so I normalized to IgG control and had a significant increase in DNA in qPCR compared to EV-myc transfected cells. POI = protein of interest.
Abcam user community
Submitted Oct 29 2010
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