ChIP abreview for Anti-Myc tag antibody - ChIP Grade

Good
Abreviews
Application
ChIP
Sample
Human Cell lysate - whole cell (Jurkat cell lines)
Specification
Jurkat cell lines
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Real-time PCR
Positive control
A stable Jurkat cell line expressing a myc- tagged version of rtTa-MYC.HBP1
Primers flanking the binding site of HBP1 on hCD2-LCR were used as a positive control
Negative control
As a negative control a Jurkat cell line that does not express the myc-tagged protein: rtTA was employed.
The HPRT gene was used as a negative control locus

Other product details

Dilution
1/100000
Incubation time
0 minute(s)

Additional data

Additional Notes
Aall values normalised to IgG and to HPRT (negative control locus)

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Submitted Nov 07 2006

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