Immunohistochemistry (Frozen sections) abreview for Anti-Myc tag antibody - ChIP Grade

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (skeleton)
Specification
skeleton
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST-BSA

Secondary antibody

Name
Non-Abcam antibody was used: Rabbit anti-Goat Cy3
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/500

Additional data

Additional Notes
Frozen tissue sections of zebrafish caudal fin stained for myc-tag. Tissue was transfected with a plasmid construct that continuously expresses beta-catenin protein tagged with myc-epitope.

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Submitted Aug 17 2007

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