Western blot abreview for Anti-Myc tag antibody - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (BY4741 strain)
Loading amount
100000 cells
Specification
BY4741 strain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C

Secondary antibody

Name
Non-Abcam antibody was used: Rabbit anti-goat from Dako
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 72 kDa
Positive control
Cells not expressing the tagged version of the protein

Additional data

Additional Notes
Predicted size of the product is 65 kDa but the actual band runs at 72 kDa.

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Submitted Oct 23 2007

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