FITC Anti-Myc tag antibody (ab1393)
Key features and details
- FITC Rabbit polyclonal to Myc tag
- Suitable for: WB, ELISA
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
FITC Anti-Myc tag antibody
See all Myc tag primary antibodies -
Description
FITC Rabbit polyclonal to Myc tag -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: WB, ELISAmore details -
Immunogen
Synthetic peptide corresponding to Myc tag. EQKLISEEDL (C-MYC) conjugated with KLH.
(Peptide available asab13837) -
General notes
Molar F/P ratio is 4.6:1
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000.
|
|
ELISA |
1/1000.
|
Notes |
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WB
1/1000 - 1/5000. |
ELISA
1/1000. |
Target
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Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
Cellular localization
Nuclear -
Alternative names
- c-myc tag antibody
- Myc Epitope Tag antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab1393 has been referenced in 4 publications.
- Najjar F et al. Role of Myocardial Infarction-Induced Neuroinflammation for Depression-Like Behavior and Heart Failure in Ovariectomized Female Rats. Neuroscience 415:201-214 (2019). PubMed: 31351141
- Najjar F et al. Sex differences in depression-like behavior and neuroinflammation in rats post-MI: role of estrogens. Am J Physiol Heart Circ Physiol 315:H1159-H1173 (2018). PubMed: 30052050
- Jain M et al. Linear assembly of a human centromere on the Y chromosome. Nat Biotechnol 36:321-323 (2018). PubMed: 29553574
- Xiao C et al. Targeting HSP70 to motoneurons protects locomotor activity from hyperthermia in Drosophila. Dev Neurobiol 67:438-55 (2007). PubMed: 17443800