Overview

  • Product name

    Anti-Myc tag antibody (FITC)
    See all Myc tag primary antibodies
  • Description

    Rabbit polyclonal to Myc tag (FITC)
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Immunogen

    EQKLISEEDL (C-MYC) conjugated with KLH.

  • General notes

    Molar F/P ratio is 4.6:1

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab1393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250. PubMed: 17443800
WB 1/1000 - 1/5000. Can be blocked with Human c-Myc peptide (ab13837).
ELISA 1/1000.

Target

  • Relevance

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization

    Nuclear
  • Alternative names

    • c-myc tag antibody
    • Myc Epitope Tag antibody

References

This product has been referenced in:

  • Xiao C  et al. Targeting HSP70 to motoneurons protects locomotor activity from hyperthermia in Drosophila. Dev Neurobiol 67:438-55 (2007). Read more (PubMed: 17443800) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (Yeast cells)
Permeabilization
No
Gating Strategy
Cells that were Myc-FITC positive
Specification
Yeast cells
Preparation
Cell harvesting/tissue preparation method: Harvested them in standard PBS with 0.1% BSA
Sample buffer: PBS with 0.1% BSA
Fixation
No fixiation

Dr. Kaushik Thakkar

Verified customer

Submitted Jul 03 2019

Answer

Thank you very much for your interest in ab1393.

Unfortunately we do not have more information about the use of the anti-Myc tag antibody (FITC) ab1393 in ICC/IF than the one available in the publication (PubMed: 17443800) and we do not currently have an image.

However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:
1. Reply to this email, letting us know you are interested in testing this product.
2. Our scientists will email you an inactive personal discount code for the value of the product.
3. Purchase and test the product at the regular price.
4. Submit an image of your results, including your discount code in the additional notes section of your Abreview.
5. Once the Abreview is submitted, the discount code will become active.
6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

About the ICC/IF protocol, we recommend the following procedure: https://www.abcam.com/index.html?pageconfig=resource&rid=11417.

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