• Product name

    Anti-Myc tag antibody (FITC)
    See all Myc tag primary antibodies
  • Description

    Chicken polyclonal to Myc tag (FITC)
  • Host species

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Immunogen

    EQKLISEEDL ( C-MYC) conjugated with KLH.

  • General notes

    Molar F/P ratio is 5.6:1


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • Purity

    IgY fraction
  • Purification notes

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • Clonality

  • Isotype

  • Light chain type

  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab1394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Can be blocked with Human c-Myc peptide (ab13837).
Flow Cyt 1/100.


  • Relevance

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization

  • Alternative names

    • c-myc tag antibody
    • Myc Epitope Tag antibody


ab1394 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for contacting us. Unfortunately, I would not recommend using a chicken antibody against a different target (such as ab1394 or ab63504), as it cannot be guaranteed that they do not react with any human antigen. I would rather suggest to use a chicken IgY polyclonal isotype control, as these isotype controls are specifically tested not to cross react. As such we currently have ab37382 available (Click here (or use the following: https://www.abcam.com/index.html?datasheet=37382).). As this antibody however is not labelled with FITC, I may recommend our EasyLink Conjugation kit (ab102883 - ab102885, see below and attached). For the compensation and set-up of the flow cytometer, I would suggest to use ab63498 to ensure the optimal settings. EasyLink antibody conjugation kits: https://www.abcam.com/easylink Click here (or use the following: https://www.abcam.com/index.html?datasheet=102883). Having said this however, I may let you know that isotype controls do not really represent a good negative control. The reasons become apparent in your particular case: The control antibody is of a different preparation ('chicken', or in case of monoclonals a different clone) and therefore may show varying binding properties compared to the specific antibody of interest (ab63498). Furthermore, as the conjugation conditions may have been different, the labelling with the fluorochrome can vary which can be a very important issue in flow cytometry. As a result of different antibody-to-fluorochrome ratios and/or different fluorochrome batches, the control antibody might indeed show a very different staining pattern, and even if it does not bind at all (as it is supposed to) can for these reasons not directly be compared to the antibody of interest. Instead, in flow cytometry another type of control is often used to determine positivity for the marker of interest. This control is termed fluorescence minus one or FMO and works like an isotype control without the actual antibody. FMO controls are set up by leaving out one of the antibodies in your staining panel. You may find more information about this type of control in the latest publications or on flow cytometry websites, such as http://www.flowcytometryuk.org/, http://www.cyto.purdue.edu/ or http://www.isac-net.org . Plus, your local flow cytometry community (core facility?) might be happy to help and advise in the use of optimal controls which are required for publishing the results. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Flow Cytometry
Saccharomyces cerevisiae Cell (Yeast expressing myc-tagged peptide)
Yeast expressing myc-tagged peptide

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Submitted May 21 2009

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