Overview

  • Product name
    Anti-Myc tag antibody [Myc.A7]
    See all Myc tag primary antibodies
  • Description
    Mouse monoclonal [Myc.A7] to Myc tag
  • Host species
    Mouse
  • Specificity
    Recognizes overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
  • Tested applications
    Suitable for: ICC/IF, ELISA, Dot blot, WB, IPmore details
  • Immunogen

    Synthetic peptide:

    EQKLISEEDL

    , corresponding to amino acids 410-419 of Human Myc.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Protein A affinity chromatography
  • Clonality
    Monoclonal
  • Clone number
    Myc.A7
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
WB 1/1000. Can be blocked with Human c-Myc peptide (ab13837).
IP Use at an assay dependent concentration.

Target

  • Relevance
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization
    Nuclear
  • Alternative names
    • c-myc tag antibody
    • Myc Epitope Tag antibody

Images

  • All lanes : Anti-Myc tag antibody [Myc.A7] (ab18185) at 1/1000 dilution

    Lane 1 : HEK293 lysate - untransfected
    Lane 2 : HEK293 lysate - transfected with Myc-tagged protein
  • Top Panel: ab18185 staining Myc Tag in rat primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-3% BSA) for 30 minutes at 20°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG (1/500) was used as secondary antibody.
    Bottom panel: Synapses (Red); Anti-myc tag (Green)

    See Abreview

References

This product has been referenced in:
  • Qiu D  et al. Sirt2-BubR1 acetylation pathway mediates the effects of advanced maternal age on oocyte quality. Aging Cell 17:N/A (2018). Mouse . Read more (PubMed: 29067790) »
  • Mabruk ZA  et al. The role of the ShcD and RET interaction in neuroblastoma survival and migration. Biochem Biophys Rep 13:99-108 (2018). Read more (PubMed: 29556564) »
See all 17 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293T + SPPL3-myc)
Gel Running Conditions
Reduced Denaturing
Loading amount
200000 cells
Specification
HEK 293T + SPPL3-myc
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Christian Ostalecki

Verified customer

Submitted Jul 20 2018

Answer

Thank you for your enquiry,

I am sorry to confirm that the Kd for ab18185 Myc tag antibody [Myc.A7] has not been assessed. It is unusual for the Kd to be measured for research grade antibodies.

We aim to provide as much information as possible to customers, and I am sorry we do not have the details you require on this occasion. If you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for getting back to me and for providing some further information about your research project.

Currently, we have 2 monoclonal antibodies against Myc tag: ab18185 and ab94936. Although they are from the same clone but from two sources and the testing conditions were different. ab94936 reacts with recombinant fragment so the other antibody ab18185 may suit your need better.

There are two specific publication for ab18185 you could take a look at to get some more information:

Biswas Ket al. A comprehensive functional characterization of BRCA2 variants associated with Fanconi anemia using mouse ES cell-based assay. Blood 118:2430-42 (2011). WB.http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=21719596&dopt=Abstract
Ohe Met al. Emi2 inhibition of the anaphase-promoting complex/cyclosome absolutely requires Emi2 binding via the C-terminal RL tail. Mol Biol Cell 21:905-13 (2010). WB.http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=20089832&dopt=Abstract



If you need any further assistance in the future, please do not hesitate to contact me.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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