Overview

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    Myc.A7
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2000.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 1.2 kDa.

Target

  • Relevance

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization

    Nuclear
  • Alternative names

    • c-myc tag antibody
    • Myc Epitope Tag antibody

Images

  • All lanes : Anti-Myc tag antibody [Myc.A7] (Biotin) (ab173829) at 1/1000 dilution

    Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
    Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
    Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
    Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
    Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg

    Secondary
    All lanes : Streptavidin-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 1.2 kDa

References

ab173829 has not yet been referenced specifically in any publications.

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