Overview

  • Product name

  • Description

    Rabbit polyclonal to MYCBP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 2425 and 2475 of Human MYCBP2 (NP_055872.3)

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab86078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 510 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      MYCBP2 belongs to the highwire family. It is a probable E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. MYCBP2 may function as a facilitator or regulator of transcriptional activation by MYC and have a role during synaptogenesis. There are two different isoforms.
    • Cellular localization

      Nuclear
    • Database links

    • Alternative names

      • AU023734 antibody
      • AW107953 antibody
      • AW546647 antibody
      • C130061D10Rik antibody
      • DKFZp686M08244 antibody
      • FLJ10106 antibody
      • FLJ13826 antibody
      • FLJ21597 antibody
      • FLJ21646 antibody
      • KIAA0916 antibody
      • MYC binding protein 2 antibody
      • PAM antibody
      • Pam, highwire, rpm 1 antibody
      • Pam/highwire/rpm-1 protein antibody
      • Phr1 antibody
      • Protein associated with Myc antibody
      • R75243 antibody
      see all

    Images

    • All lanes : Anti-MYCBP2 antibody (ab86078) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Predicted band size: 510 kDa



      Detection: chemiluminescence with exposure time of 3 seconds

    References

    This product has been referenced in:

    • Liang J  et al. Epigenetically regulated miR-1247 functions as a novel tumour suppressor via MYCBP2 in methylator colon cancers. Br J Cancer 119:1267-1277 (2018). Read more (PubMed: 30318507) »
    • Carter EL  et al. High Affinity Heme Binding to a Heme Regulatory Motif on the Nuclear Receptor Rev-erbß Leads to Its Degradation and Indirectly Regulates Its Interaction with Nuclear Receptor Corepressor. J Biol Chem 291:2196-222 (2016). Read more (PubMed: 26670607) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    5 µg
    Gel Running Conditions
    Reduced Denaturing (6% gel)
    Sample
    Mouse Cell lysate - whole cell (mouse ES cells)
    Specification
    mouse ES cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Nov 11 2013

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