Overview

  • Product name
    Anti-Mycobacterium antibody
  • Description
    Rabbit polyclonal to Mycobacterium
  • Host species
    Rabbit
  • Specificity
    Reacts primarily against lipoarabinomanna (LAM).
  • Tested applications
    Suitable for: ELISAmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with all mycobacterium species tested (M. bovis, M. scrofuleceum, M. kansaii, M. avium/intracellulare, M. cheloni). Demonstrates slight reactivity with Rhidococcus and Nocardia.
  • Immunogen

    Genus specific antigens extracted from Mycobacterium tuberculosis.

Properties

Applications

Our Abpromise guarantee covers the use of ab20832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Relevance
      Mycobacterium is a genus of actinobacteria, given its own family, the Mycobacteriaceae. It includes many pathogens known to cause serious diseases in mammals, including tuberculosis and leprosy. Mycobacteria can be classified into several major groups for purpose of diagnosis and treatment: M. tuberculosis complex which can cause tuberculosis: M. tuberculosis, M. bovis, M. africanum, and M. micoti. M. leprae which causes Hansen's disease or leprosy. Nontuberculous mycobacteria (NTM) are all the other mycobacteria which can cause pulmonary disease resembling tuberculosis, lymphadenitis, skin disease, or disseminated disease.

    References

    This product has been referenced in:
    • Ufimtseva E  et al. Ex vivo expansion of alveolar macrophages with Mycobacterium tuberculosis from the resected lungs of patients with pulmonary tuberculosis. PLoS One 13:e0191918 (2018). Read more (PubMed: 29401466) »
    • Ufimtseva E Differences between Mycobacterium-Host Cell Relationships in Latent Tuberculous Infection of Mice Ex Vivo and Mycobacterial Infection of Mouse Cells In Vitro. J Immunol Res 2016:4325646 (2016). Read more (PubMed: 27066505) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Answer

    Thank you for your enquiry. I can confirm that the immunogen for ab20832 Mycobacterium antibody was a cell wall extract of M. tuberculosis. The exact proteins in the immunogen were not characterized. This antibody reacts primarily against lipoarabinomannan (LAM), but also shows reactivity to other mycobacterium proteins. This is a polyclonal antibody and therefore recognizes many epitopes on several proteins. I am sorry the epitopes have not been mapped. Unfortunately, we are not aware of a published reference using this antibody. Any we are aware of would be listed on the datasheet. I hope this information is helpful to you. Should you have any further questions, please do not hesitate to contact us.

    Read More
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin, Colon, Tonsils)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9, 20 min
    Permeabilization
    Yes - Bond
    Specification
    Skin, Colon, Tonsils
    Blocking step
    Levamisol in Mixed Red as blocking agent for 15 minute(s) · Concentration: 10%
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Oct 13 2015

    Answer

    Thank you for contacting us.

    The concnetration has not been determined for this antibody as it is provided as whole antiserum. It thus contains other proteins and serum IgGs that would interfere with a protein assay for the specific antibody.

    A rough estimate for the concnentraion of the specific IgG in serum in general is max. 1 mg/ml (10% of the total IgG conc) at best.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Answer

    Thank you for contacting us. I am sorry for the delay in getting back to you.

    As requested, I have generated the discount code for the Anti-Mycobacterium antibody (ab20832) as follows:

    Discount code: xxxxxxxxxx

    Expiration date: 10th January 2013

    Please go ahead and purchase the antibodies ab20832 and ab97068 as normal. Once you have carried out your experiment, please submit an Abreview forab20832 (regardless of if the results are positive or negative) and when filling in the form please include the discount code above in the "additional comments" section of the form. Once the Abreview has been submitted the code can then be used to claim your free primary antibody (or the equivalent price of ab20832 off any of the products in our catalogue). The code must be used before the expiration date specified above.

    More information on the discount code scheme can be found from the following link:

    www.abcam.com/collaborationdiscount

    I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

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    Answer

    Thank you for your reply.

    Unfortunately, due to the size of our catalogue and the logistics that would be required we are not able to provide sample sizes of any of our products. This is why we offer the testing discount scheme to our customers. For the Abreview, you would only need to provide a brief out like of the protocol used and an image of the experiment results. we would not require any in depth detail of the experiment. An example of an Abreview can be viewed from the following link:

    https://www.abcam.com/index.html?datasheet=20920&tab=abreviews&intabreviewid=7395

    I hope this information has been of help. If you require any further information or you would like to participate in the discount scheme, please do let me know.

    Read More

    Answer

    Thank you for confirming those details.

    You may want to consider the following antibodies:

    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-FITC-pre-adsorbed-ab97068.html https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-FITC-pre-adsorbed-ab97068.html

    https://www.abcam.com/Donkey-F-ab-2-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-PE-pre-adsorbed-ab7007.html https://www.abcam.com/Donkey-F-ab-2-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-PE-pre-adsorbed-ab7007.html

    The Anti-Mycobacterium antibody (ab20832) has as yet only been used in ELISAs. This does not mean that it would not work in other applications, simply that we have not tested it ourselves and would therefore not be able to guarantee its performance.

    However, if you would like to purchase ab20832 and use it with your samples measured by flow cytometry, we can offer you a testing discount. This offer means that if you purchase ab20832 as normal, test the antibody and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab20832 off any product). More information on this offer can be found here:

    www.abcam.com/collaborationdiscount

    Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

    If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab20832.

    I have noticed that you tend to reply using the website contact form. Please feel free to reply directly to my emails, this means they will more likely be directed to me and I will know what the case is about.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Answer

    Thank you for your reply.

    We have several florescent conjugated secondary antibodies which may be suitable for use with the Anti-Mycobacterium antibody (ab20832). In order to advise you, could you let me know which application you are hoping to use the antibodies in and if you have a preference of the fluorescent conjugate or an emission/excitation range you are interested in.

    Once I have confirmation of this I can provide you with a few suggestions. I look forward to receiving your reply.

    Read More

    Answer

    Thank you for contacting us and your interest in our products.

    We have several antibodies which may be used with the Anti-Mycobacterium antibody (ab20832) and the one most suitable for you would depend on the experiment you are hoping to perform. We have a secondary antibody search engine which allows you to stipulate a number of parameters to find themost suitable secondary antibodies for you. Here is a link to the search engine:

    https://www.abcam.com/index.html?pageconfig=productmap&cl=918

    If you wanted to use ab20832 in an ELISA with HRP as the conjugate, the following antibodies may be suitable:

    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-HRP-pre-adsorbed-ab97080.html https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-HRP-pre-adsorbed-ab97080.html

    or

    https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-HRP-pre-adsorbed-ab98503.html https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-HRP-pre-adsorbed-ab98503.html

    I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

    Read More

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