• Product name
    Anti-Mycobacterium antibody
  • Description
    Rabbit polyclonal to Mycobacterium
  • Host species
  • Specificity
    Reacts primarily against lipoarabinomanna (LAM).
  • Tested applications
    Suitable for: ELISAmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with all mycobacterium species tested (M. bovis, M. scrofuleceum, M. kansaii, M. avium/intracellulare, M. cheloni). Demonstrates slight reactivity with Rhidococcus and Nocardia.
  • Immunogen

    Genus specific antigens extracted from Mycobacterium tuberculosis.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Proclin 300
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab20832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Relevance
      Mycobacterium is a genus of actinobacteria, given its own family, the Mycobacteriaceae. It includes many pathogens known to cause serious diseases in mammals, including tuberculosis and leprosy. Mycobacteria can be classified into several major groups for purpose of diagnosis and treatment: M. tuberculosis complex which can cause tuberculosis: M. tuberculosis, M. bovis, M. africanum, and M. micoti. M. leprae which causes Hansen's disease or leprosy. Nontuberculous mycobacteria (NTM) are all the other mycobacteria which can cause pulmonary disease resembling tuberculosis, lymphadenitis, skin disease, or disseminated disease.


    This product has been referenced in:
    • Ufimtseva E  et al. Ex vivo expansion of alveolar macrophages with Mycobacterium tuberculosis from the resected lungs of patients with pulmonary tuberculosis. PLoS One 13:e0191918 (2018). Read more (PubMed: 29401466) »
    • Ufimtseva E Differences between Mycobacterium-Host Cell Relationships in Latent Tuberculous Infection of Mice Ex Vivo and Mycobacterial Infection of Mouse Cells In Vitro. J Immunol Res 2016:4325646 (2016). Read more (PubMed: 27066505) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry. I can confirm that the immunogen for ab20832 Mycobacterium antibody was a cell wall extract of M. tuberculosis. The exact proteins in the immunogen were not characterized. This antibody reacts primarily against lipoarabi...

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Human Tissue sections (Skin, Colon, Tonsils)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9, 20 min
    Yes - Bond
    Skin, Colon, Tonsils
    Blocking step
    Levamisol in Mixed Red as blocking agent for 15 minute(s) · Concentration: 10%

    Abcam user community

    Verified customer

    Submitted Oct 13 2015

    Thank you for contacting us.

    The concnetration has not been determined for this antibody as it is provided as whole antiserum. It thus contains other proteins and serum IgGs that would interfere with a protein assay for the specific antibody.<...

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    Thank you for contacting us. I am sorry for the delay in getting back to you.

    As requested, I have generated the discount code for the Anti-Mycobacterium antibody (ab20832) as follows:

    Discount code: xxxxxxxxxx

    Expiration d...

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    Thank you for your reply.

    Unfortunately, due to the size of our catalogue and the logistics that would be required we are not able to provide sample sizes of any of our products. This is why we offer the testing discount scheme to our custome...

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    Thank you for confirming those details.

    You may want to consider the following antibodies:

    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-FITC-pre-adsorbed-ab97068.html https://www.abcam.com/Goat-polyclon...

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    Thank you for your reply.

    We have several florescent conjugated secondary antibodies which may be suitable for use with the Anti-Mycobacterium antibody (ab20832). In order to advise you, could you let me know which application you are hoping ...

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    Thank you for contacting us and your interest in our products.

    We have several antibodies which may be used with the Anti-Mycobacterium antibody (ab20832) and the one most suitable for you would depend on the experiment you are hoping to perf...

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