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Anti-Mycobacterium L-alanine dehydrogenase antibody [WHO no. IT-46] (ab51804)

Reviews (1) Submit a question References (1)

Key features and details

  • Mouse monoclonal [WHO no. IT-46] to Mycobacterium L-alanine dehydrogenase
  • Suitable for: WB, ELISA
  • Isotype: IgG1

Overview

  • Product name

    Anti-Mycobacterium L-alanine dehydrogenase antibody [WHO no. IT-46]

  • Description

    Mouse monoclonal [WHO no. IT-46] to Mycobacterium L-alanine dehydrogenase

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Tissue, cells or virus corresponding to Mycobacterium L-alanine dehydrogenase.

  • Epitope

    Recognizes the 12 aa SAITDADFKAAG (aa 45-56) region of Ald of Mycobacterium tuberculosis.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use at an assay dependent dilution. Predicted molecular weight: 40 kDa.
ELISA
Use at an assay dependent dilution.
Notes
WB
Use at an assay dependent dilution. Predicted molecular weight: 40 kDa.
ELISA
Use at an assay dependent dilution.

Target

  • Relevance

    Mycobacterium L-alanine dehydrogenase (ald) belongs to the AlaDH/PNT family and is a 40 kDa secreted protein from short term culture of Mycobacterium tuberculosis; which may play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer.

  • Cellular localization

    Secreted

  • Alternative names

    • 40 kDa antigen antibody
    • Alanine dehydrogenase antibody
    • ald antibody
    • TB43 antibody

References (1)

Publishing research using ab51804? Please let us know so that we can cite the reference in this datasheet.

ab51804 has been referenced in 1 publication.

  • Hutter B & Singh M Host vector system for high-level expression and purification of recombinant, enzymatically active alanine dehydrogenase of Mycobacterium tuberculosis. Gene 212:21-9 (1998). PubMed: 9661660

Customer reviews and Q&As

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Western blot abreview for Anti-Mycobacterium L-alanine dehydrogenase antibody [WHO no. IT-46]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mycobacterium tuberculosis Cell lysate - whole cell (Mycobacterium tuberculosis H37Rv)
Loading amount
40 µg
Specification
Mycobacterium tuberculosis H37Rv
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Lay Harn Gam

Verified customer

Submitted May 06 2009

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