Anti-Mycobacterium tuberculosis Ag85 antibody [HYT27] (ab36731)
Key features and details
- Mouse monoclonal [HYT27] to Mycobacterium tuberculosis Ag85
- Suitable for: ELISA, WB
- Isotype: IgG1
Overview
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Product name
Anti-Mycobacterium tuberculosis Ag85 antibody [HYT27] -
Description
Mouse monoclonal [HYT27] to Mycobacterium tuberculosis Ag85 -
Host species
Mouse -
Specificity
This antibody is specific for Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein. No cross-reactivity to antigens from Listeria monocytogenes or Escherichia coli. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Mycobacterium tuberculosis
Does not react with: Escherichia coli -
Immunogen
Tissue, cells or virus corresponding to Mycobacterium tuberculosis Ag85.
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Epitope
Epitope is lacking in PPD (tuberculin purified protein derivative, derived from culture filtrate, heat treated and precipitated with trichloroacetic acid). -
General notes
WHO no. IT-49
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
HYT27 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/400.
Cell culture filtrate or whole cells directly coated onto microtitre wells can be used. |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 31,39 kDa.
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Notes |
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ELISA
1/400. Cell culture filtrate or whole cells directly coated onto microtitre wells can be used. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 31,39 kDa. |
Target
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Relevance
Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly. -
Alternative names
- Antigen 85 complex A antibody
- Antigen 85 complex B antibody
- Antigen 85 complex C antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab36731 has been referenced in 6 publications.
- Queval CJ et al. Macrophage-specific responses to human- and animal-adapted tubercle bacilli reveal pathogen and host factors driving multinucleated cell formation. PLoS Pathog 17:e1009410 (2021). PubMed: 33720986
- Chai Q et al. A Mycobacterium tuberculosis surface protein recruits ubiquitin to trigger host xenophagy. Nat Commun 10:1973 (2019). PubMed: 31036822
- Solans L et al. The PhoP-dependent ncRNA Mcr7 modulates the TAT secretion system in Mycobacterium tuberculosis. PLoS Pathog 10:e1004183 (2014). WB . PubMed: 24874799
- Rengarajan J et al. Mycobacterium tuberculosis Rv2224c modulates innate immune responses. Proc Natl Acad Sci U S A 105:264-9 (2008). WB . PubMed: 18172199
- Worsaae A et al. Allergenic and blastogenic reactivity of three antigens from Mycobacterium tuberculosis in sensitized guinea pigs. Infect Immun 55:2922-7 (1987). PubMed: 3119491
- Andersen AB et al. Interspecies reactivity of five monoclonal antibodies to Mycobacterium tuberculosis as examined by immunoblotting and enzyme-linked immunosorbent assay. J Clin Microbiol 23:446-51 (1986). PubMed: 2420818