• Product name

    Anti-Mycobacterium tuberculosis Ag85 antibody
  • Description

    Mouse monoclonal to Mycobacterium tuberculosis Ag85
  • Host species

  • Specificity

    This antibody is specific for Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein. No cross-reactivity to antigens from Listeria monocytogenes or Escherichia coli.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Other species
    Does not react with: Escherichia coli
  • Immunogen

    Bacterial press extract from Mycobacterium tuberculosis H37Rv

  • Epitope

    Epitope is lacking in PPD (tuberculin purified protein derivative, derived from culture filtrate, heat treated and precipitated with trichloroacetic acid).
  • General notes

    WHO no. IT-49


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 2.9% Sodium chloride, 0.0268% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

  • Myeloma

  • Isotype

  • Light chain type

  • Research areas


Our Abpromise guarantee covers the use of ab36731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/400. Cell culture filtrate or whole cells directly coated onto microtitre wells can be used.
WB Use at an assay dependent dilution. Predicted molecular weight: 18,31,39 kDa.


  • Relevance

    Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly.
  • Alternative names

    • Antigen 85 complex A antibody
    • Antigen 85 complex B antibody
    • Antigen 85 complex C antibody
    • fbpA antibody
    • fbpB antibody
    • fbpC antibody
    • Secreted antigen 85 A antibody
    • Secreted antigen 85 B antibody
    • Secreted antigen 85 C antibody
    see all


This product has been referenced in:

  • Chai Q  et al. A Mycobacterium tuberculosis surface protein recruits ubiquitin to trigger host xenophagy. Nat Commun 10:1973 (2019). Read more (PubMed: 31036822) »
  • Solans L  et al. The PhoP-dependent ncRNA Mcr7 modulates the TAT secretion system in Mycobacterium tuberculosis. PLoS Pathog 10:e1004183 (2014). WB . Read more (PubMed: 24874799) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


HYT 27, does react in WB with both Ag85A, B and C, see the paper by Armitige LY and colleagues, whom used HYT 27 in WB (on page 774, fbpA antibody) and showed very good reaction with A.

Armitage et al., INFECTION AND IMMUNITY, Feb. 2000, Vol. 68, No. 2 p. 767–778.

Read More


Thank you for contacting us.

I have had the opportunity to speak with the lab regarding the specificity and cross reactivity of ab36731.


HYT 27 is specific for Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein.
No cross-reactivity to antigens from Listeria monocytogenes or Escherichia coli.


HYT 27 reacts strongly in ELISA with M. tuberculosis cell culture filtrate or whole cells directly coated onto the microtiter wells.
In Western blotting after SDS-PAGE, HYT 27 reacts strongly with Ag85C and weaker with Ag85A and Ag85B.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More


Thank you for your enquiry. I regret to inform you that the originator of this antibody no longer has sufficient stocks for us to be able to make this product available to our customers. We have therefore been forced to withdraw ab34957 from our product range. I do hope that this does not cause you too much inconvenience. I have conducted a search for you and found some alternative antibodies which may suit your need: - Unlabelled: ab43019 ab36731 ab905 ab20962 - Biotin-conjugated: ab20027 - it has only been tested in IHC-P I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More
This product is known to not work in this application or species.
Western blot
Escherichia coli Purified protein (transformed E coli)
Loading amount
0.01 µg
transformed E coli
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 29 2007

For licensing inquiries, please contact partnerships@abcam.com

Sign up