Key features and details
- Mouse monoclonal to Mycobacterium tuberculosis Ag85
- Suitable for: ELISA, WB
- Isotype: IgG1
Product nameAnti-Mycobacterium tuberculosis Ag85 antibody
DescriptionMouse monoclonal to Mycobacterium tuberculosis Ag85
SpecificityThis antibody is specific for Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein. No cross-reactivity to antigens from Listeria monocytogenes or Escherichia coli.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Other species
Does not react with: Escherichia coli
Tissue, cells or virus corresponding to Mycobacterium tuberculosis Ag85.
EpitopeEpitope is lacking in PPD (tuberculin purified protein derivative, derived from culture filtrate, heat treated and precipitated with trichloroacetic acid).
WHO no. IT-49
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab36731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/400. Cell culture filtrate or whole cells directly coated onto microtitre wells can be used.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 31,39 kDa.|
RelevanceMycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly.
- Antigen 85 complex A antibody
- Antigen 85 complex B antibody
- Antigen 85 complex C antibody
ab36731 has been referenced in 5 publications.
- Chai Q et al. A Mycobacterium tuberculosis surface protein recruits ubiquitin to trigger host xenophagy. Nat Commun 10:1973 (2019). PubMed: 31036822
- Solans L et al. The PhoP-dependent ncRNA Mcr7 modulates the TAT secretion system in Mycobacterium tuberculosis. PLoS Pathog 10:e1004183 (2014). WB . PubMed: 24874799
- Rengarajan J et al. Mycobacterium tuberculosis Rv2224c modulates innate immune responses. Proc Natl Acad Sci U S A 105:264-9 (2008). WB . PubMed: 18172199
- Worsaae A et al. Allergenic and blastogenic reactivity of three antigens from Mycobacterium tuberculosis in sensitized guinea pigs. Infect Immun 55:2922-7 (1987). PubMed: 3119491
- Andersen AB et al. Interspecies reactivity of five monoclonal antibodies to Mycobacterium tuberculosis as examined by immunoblotting and enzyme-linked immunosorbent assay. J Clin Microbiol 23:446-51 (1986). PubMed: 2420818