Anti-Mycobacterium tuberculosis Ag85 antibody (ab36731)

Overview

  • Product name
    Anti-Mycobacterium tuberculosis Ag85 antibody
  • Description
    Mouse monoclonal to Mycobacterium tuberculosis Ag85
  • Host species
    Mouse
  • Specificity
    This antibody is specific for Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein. No cross-reactivity to antigens from Listeria monocytogenes or Escherichia coli.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Other species
    Does not react with: Escherichia coli
  • Immunogen

    Bacterial press extract from Mycobacterium tuberculosis H37Rv

  • Epitope
    Epitope is lacking in PPD (tuberculin purified protein derivative, derived from culture filtrate, heat treated and precipitated with trichloroacetic acid).
  • General notes

    WHO no. IT-49

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 2.9% Sodium chloride, 0.0268% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/400. Cell culture filtrate or whole cells directly coated onto microtitre wells can be used.
WB Use at an assay dependent dilution. Predicted molecular weight: 18,31,39 kDa.

Target

  • Relevance
    Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly.
  • Alternative names
    • Antigen 85 complex A antibody
    • Antigen 85 complex B antibody
    • Antigen 85 complex C antibody
    • fbpA antibody
    • fbpB antibody
    • fbpC antibody
    • Secreted antigen 85 A antibody
    • Secreted antigen 85 B antibody
    • Secreted antigen 85 C antibody
    see all

References

This product has been referenced in:
  • Solans L  et al. The PhoP-dependent ncRNA Mcr7 modulates the TAT secretion system in Mycobacterium tuberculosis. PLoS Pathog 10:e1004183 (2014). WB . Read more (PubMed: 24874799) »
  • Rengarajan J  et al. Mycobacterium tuberculosis Rv2224c modulates innate immune responses. Proc Natl Acad Sci U S A 105:264-9 (2008). WB . Read more (PubMed: 18172199) »

See all 4 Publications for this product

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (transformed E coli)
Loading amount
0.01 µg
Specification
transformed E coli
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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Verified customer

Submitted Aug 29 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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