Overview

  • Product name
    Anti-Mycobacterium tuberculosis antibody
    See all Mycobacterium tuberculosis primary antibodies
  • Description
    Rabbit polyclonal to Mycobacterium tuberculosis
  • Host species
    Rabbit
  • Specificity
    This antibody is reactive with other Mycobacteria species including: M. avium, M. phlei, and M. parafortuitum. This antibody has been reported not to be reactive with E. coli K12, Salmonella typhimurium, Pseudomonas aeruginosa, Streptococcus (group B), Candida albicans and Neisseria meningitides.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
  • Immunogen

    Purified Protein Derivative

  • Positive control
    • Infected lung

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, Carrier protein, Da Vinci Green Diluent, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24475192
IHC-P 1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. ABC method.
IHC-Fr 1/100 - 1/200.

ABC method.

Target

  • Relevance
    Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
  • Alternative names
    • M. tuberculosis antibody

Images

  • Lung tissue stained with ab905 at 1/500.

References

This product has been referenced in:
  • Parasa VR  et al. Evaluation of the immunogenic capability of the BCG strains BCG?BCG1419c and BCG?BCG1416c in a three-dimensional human lung tissue model. Vaccine 36:1811-1815 (2018). Read more (PubMed: 29475763) »
  • Kayanuma H  et al. Disseminated nontuberculous mycobacterial disease in a cat caused by Mycobacterium sp. strain MFM001. Vet Microbiol 220:90-96 (2018). Read more (PubMed: 29885807) »
See all 10 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Answer

Thank you for your enquiry.

I am sorry to confirm that after further investigation with our source, I can confirm that ab20962 has not specifically been tested for detection of BCG.

If they would like totryit for detection of BCG, I would be pleased to providea testing discount. Please let me know if theyare interested.

If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for contacting us.

This is a polyclonal antibody that was produced using PPD (Purified Protein Derivative) from M. tuberculosis. PPD is a collection of soluble proteins secreted into the culture medium when M. tuberculosis is grown. Thus, there are multiple to which this antibody was raised and react with. Also, because this is a polyclonal antibody, it will react with multiple epitopes on each of the proteins.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your inquiry.

ab905 is a polyclonal antibody that was produced using PPD (Purified Protein Derivative)from M. tuberculosis.

PPD is a collection of soluble proteins secreted into the culture medium when M. tuberculosis is grown.

Thus, there are multipleproteinsto which this antibody was raised and reacts with. Also, because this is a polyclonal antibody, it will react with multiple epitopes on each of the proteins.

Therefore I have to confirm that ab905 will not specifically stain the cell wall.

I hope this information is helpful.

Read More

Answer

Thank you for your enquiry. I regret to inform you that the originator of this antibody no longer has sufficient stocks for us to be able to make this product available to our customers. We have therefore been forced to withdraw ab34957 from our product range. I do hope that this does not cause you too much inconvenience. I have conducted a search for you and found some alternative antibodies which may suit your need: - Unlabelled: ab43019 ab36731 ab905 ab20962 - Biotin-conjugated: ab20027 - it has only been tested in IHC-P I hope this helps and if I can assist further, please do not hesitate to contact me.

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Answer

Thank you for contacting us.  I apologize for the delay in my reply.  The Mycobacterium tuberculosis antibody ab905 is also reactive with M. avium, M. phlei, and M. parafortuitum.  This antibody does not react with E. coli K12, Salmonella typhimurium, Pseudomonas aeruginosa, Streptococcus (group B), Candida albicans or Neisseria meningitides.  The immunogen for ab905 was a Purified Protein Derivative (PPD) of Mycobacterium tuberculosis.  I hope this helps, please let me know if you need any additional information. 

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Question
Answer

Thank you for your enquiry. Unfortunately we have no information as to whether the antibody cross-reacts with that strain. I am sorry I could not be more helpful. Please do not hesitate to contact us if you need anything further.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Inflamed synovium from patients (osteoarthritis))
Specification
Inflamed synovium from patients (osteoarthritis)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.05% BSA

Dr. Dan McWilliams

Verified customer

Submitted Nov 28 2005

Answer

Thank you for explaining your details to me. I now understand why you would choose to use a anti-TB antibody on mammalian cells. Following your comprehensive experiments it appears that there is clear reactivity against a human target protein by this antiserum. The positive control that we would recommend is an lung infected with TB. However, unfortunately I do not have details of the species that this would have been tested on. On this occasion this antiserum has not performed as detailed on the datasheet. Therefore I would like to offer you a credit note. Before I can do this please can you e-mail me the date of purchase and the original purchase order number. Please can I encourage you to submit an Abreview of this product by navigating to the product datasheet and clicking on the "Abreviews" tab. I look forward to hearing from you.

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Answer

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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