Anti-Mycobacterium tuberculosis antibody (ab905)

Reviews (1)Q&A (8)References (10)

Overview

  • Product name
    Anti-Mycobacterium tuberculosis antibody
    See all Mycobacterium tuberculosis primary antibodies
  • Description
    Rabbit polyclonal to Mycobacterium tuberculosis
  • Host species
    Rabbit
  • Specificity
    This antibody is reactive with other Mycobacteria species including: M. avium, M. phlei, and M. parafortuitum. This antibody has been reported not to be reactive with E. coli K12, Salmonella typhimurium, Pseudomonas aeruginosa, Streptococcus (group B), Candida albicans and Neisseria meningitides.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
  • Immunogen

    Purified Protein Derivative

  • Positive control
    • Infected lung

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, Carrier protein, Da Vinci Green Diluent, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24475192
IHC-P 1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. ABC method.
IHC-Fr 1/100 - 1/200.

ABC method.

Target

  • Relevance
    Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
  • Alternative names
    • M. tuberculosis antibody

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mycobacterium tuberculosis antibody (ab905)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mycobacterium tuberculosis antibody (ab905)
    Lung tissue stained with ab905 at 1/500.

References

This product has been referenced in:
  • Parasa VR  et al. Evaluation of the immunogenic capability of the BCG strains BCG?BCG1419c and BCG?BCG1416c in a three-dimensional human lung tissue model. Vaccine 36:1811-1815 (2018). Read more (PubMed: 29475763) »
  • Kayanuma H  et al. Disseminated nontuberculous mycobacterial disease in a cat caused by Mycobacterium sp. strain MFM001. Vet Microbiol 220:90-96 (2018). Read more (PubMed: 29885807) »
See all 10 Publications for this product

Publishing research using ab905? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-9 of 9 Abreviews or Q&A

Question

Our customer is looking for anti-BCG antibody for ICC/IF.

Her sample is BCG infected human tumor cell line.

I found that you recommended ab20962 as BCG antibody in scientific support Q2 of ab905.

https://www.abcam.com/Mycobacterium-tuberculosis-antibody-ab905-support.html

So I would like to know if it is possible to use mycobacterium tuberculosis antibody instead of BCG antibody.

Please let me know.

Best regards

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Answer

Thank you for your enquiry.

I am sorry to confirm that after further investigation with our source, I can confirm that ab20962 has not specifically been tested for detection of BCG.

If they would like totryit for detection of BCG, I would be pleased to providea testing discount. Please let me know if theyare interested.

If you have any further questions, please do not hesitate to contact us.

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Question

I am interested in the product ab905, an anti-mycobacterium tuberculosis antibody. Your website mentions that it is specific to a purified protein derivative. I was wondering whether you would be able to give me more information about this protein i.e what the protein is and the normal location of this protein?
Thank you

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Answer

Thank you for contacting us.

This is a polyclonal antibody that was produced using PPD (Purified Protein Derivative) from M. tuberculosis. PPD is a collection of soluble proteins secreted into the culture medium when M. tuberculosis is grown. Thus, there are multiple to which this antibody was raised and react with. Also, because this is a polyclonal antibody, it will react with multiple epitopes on each of the proteins.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Product code: 905

Inquiry: I want to use the above mentioned antibody for stainnig mycobacterium tuberculosis cell wall and then want to do imaging using confocal. So, i wanted to know is this antibody is against Mtb cell wall protein.

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Answer

Thank you for your inquiry.

ab905 is a polyclonal antibody that was produced using PPD (Purified Protein Derivative)from M. tuberculosis.

PPD is a collection of soluble proteins secreted into the culture medium when M. tuberculosis is grown.

Thus, there are multipleproteinsto which this antibody was raised and reacts with. Also, because this is a polyclonal antibody, it will react with multiple epitopes on each of the proteins.

Therefore I have to confirm that ab905 will not specifically stain the cell wall.

I hope this information is helpful.

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Question

Greetings,   I´m a PhD student in Health Sciences at the University of Minho (Portugal) and I need a biotin conjugated monoclonal antibody for Mycobacterium bovis BCG. I would like to know if you have this antibody and, if so, what product would you recommend me for my research. Could you also inform me about the price and availability of this antibody? You used to have a BCG antibody with the code ab34957, but I can no longer find it. Have you got any option? Thank you for your time and consideration. Best regards,  

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Answer

Thank you for your enquiry. I regret to inform you that the originator of this antibody no longer has sufficient stocks for us to be able to make this product available to our customers. We have therefore been forced to withdraw ab34957 from our product range. I do hope that this does not cause you too much inconvenience. I have conducted a search for you and found some alternative antibodies which may suit your need: - Unlabelled: ab43019 ab36731 ab905 ab20962 - Biotin-conjugated: ab20027 - it has only been tested in IHC-P I hope this helps and if I can assist further, please do not hesitate to contact me.

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Question

Has this antibody been tested for cross-reactivity with other species of mycobacterium? Can you please tell me what purified PPD is?

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Answer

Thank you for contacting us.  I apologize for the delay in my reply.  The Mycobacterium tuberculosis antibody ab905 is also reactive with M. avium, M. phlei, and M. parafortuitum.  This antibody does not react with E. coli K12, Salmonella typhimurium, Pseudomonas aeruginosa, Streptococcus (group B), Candida albicans or Neisseria meningitides.  The immunogen for ab905 was a Purified Protein Derivative (PPD) of Mycobacterium tuberculosis.  I hope this helps, please let me know if you need any additional information. 

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Question

Has this antibody been tested on strain h37Ra (ATCC 25177)?

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Answer

Thank you for your enquiry. Unfortunately we have no information as to whether the antibody cross-reacts with that strain. I am sorry I could not be more helpful. Please do not hesitate to contact us if you need anything further.

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Immunohistochemistry (Frozen sections) abreview for Anti-Mycobacterium tuberculosis antibody

 Inconclusive
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Inflamed synovium from patients (osteoarthritis))
Specification
Inflamed synovium from patients (osteoarthritis)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.05% BSA
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Dr. Dan McWilliams

Verified customer

Submitted Nov 28 2005

Question

We were asked by academic referees from Arthritis and Rheumatism to show that our rabbit anti-humanTrpV1 antibodies were not binding to the synovium non-specifically. We chose an "irrelevant" target for human synovium of a rabbit polyclonal antibody directed against an antigen that we did not expect to see in the synovium. Your ab905 was used at the same concentration as our TrpV1 antibody and bound to more targets than TrpV1 (see pictures). Normally, anti-TB, fails to bind to any cells in the synovium (although we do get mast cell peroxidase staining - which is resistant to methanol:h2O2 blocking). Our group has used other anti-TB antibodies for "irrelevant" controls in the past. In other words, we were expecting to see no staining in the synovium, which would confirm that our anti-TrpV1 staining was due to TrpV1 antigen being present. We still believe that TrpV1 antigen is present in the synovium, however, we do not believe that mycobacterium tuberculosi are hiding in the knee! We believe that ab905 is binding to another target in our immunohistochemistry studies. It can clearly to seen in blood vessels and other non-mast cell types. NB. We did parallel experiments in the inflamed rat synovium. Goat anti-E.coli was our irrelevant antibody (I believe it was ab13627), and it did not bind to non-specific targets. Why do you need to know my motivation for using ab905 in the synovium?

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Answer

Thank you for explaining your details to me. I now understand why you would choose to use a anti-TB antibody on mammalian cells. Following your comprehensive experiments it appears that there is clear reactivity against a human target protein by this antiserum. The positive control that we would recommend is an lung infected with TB. However, unfortunately I do not have details of the species that this would have been tested on. On this occasion this antiserum has not performed as detailed on the datasheet. Therefore I would like to offer you a credit note. Before I can do this please can you e-mail me the date of purchase and the original purchase order number. Please can I encourage you to submit an Abreview of this product by navigating to the product datasheet and clicking on the "Abreviews" tab. I look forward to hearing from you.

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Question

We are writing you from Turkey. We have not found any distributor of your company in TURKEY. We are working on Mycobacterial infections in both captive and wild pheasants. We want to buy your primary antibody against Mycobacterium. But we wonder that whether your antibody works on pheasants immunohistochemically. so, is it possible for us to get small amount of tester solution of that antibody mentioned above? Antibody information: ab905 your cataloque number

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Answer

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

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