Key features and details
- Rabbit polyclonal to Mycobacterium tuberculosis (FITC)
- Suitable for: IHC-P, ICC/IF
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Product nameAnti-Mycobacterium tuberculosis antibody (FITC)
See all Mycobacterium tuberculosis primary antibodies
DescriptionRabbit polyclonal to Mycobacterium tuberculosis (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
The antibody is covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab20962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50.|
|ICC/IF||Use at an assay dependent dilution. Acetone fixation is recommended.|
RelevanceMycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
- M. tuberculosis antibody
ab20962 has been referenced in 4 publications.
- Ordonez AA et al. Radiosynthesis and PET Bioimaging of 76Br-Bedaquiline in a Murine Model of Tuberculosis. ACS Infect Dis N/A:N/A (2019). PubMed: 31345032
- Bouttier M et al. Alu repeats as transcriptional regulatory platforms in macrophage responses to M. tuberculosis infection. Nucleic Acids Res N/A:N/A (2016). Human . PubMed: 27604870
- Verway M et al. Vitamin D induces interleukin-1ß expression: paracrine macrophage epithelial signaling controls M. tuberculosis infection. PLoS Pathog 9:e1003407 (2013). ICC/IF ; Mouse . PubMed: 23762029
- Brzostek A et al. Mycobacterium tuberculosis is able to accumulate and utilize cholesterol. J Bacteriol 191:6584-91 (2009). PubMed: 19717592