Overview

  • Product name

    Anti-MyD88 antibody [4D6]
    See all MyD88 primary antibodies
  • Description

    Mouse monoclonal [4D6] to MyD88
  • Host species

    Mouse
  • Specificity

    Espected to recognise Isoforms 1, 3, 4, 5, CRAa and CRAb.
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 50-100 of Human MyD88 (NP_002459).

  • Positive control

    • human ovary, human prostate and Jurkat cell lysates and CF-7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab107585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 33 kDa.
Flow Cyt Use 0.5µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
  • Sequence similarities

    Contains 1 death domain.
    Contains 1 TIR domain.
  • Domain

    The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Mutant myeloid differentiation primary response 88 antibody
    • MYD 88 antibody
    • Myd88 antibody
    • MYD88_HUMAN antibody
    • MYD88D antibody
    • Myeloid differentiation marker 88 antibody
    • Myeloid differentiation primary response 88 antibody
    • Myeloid differentiation primary response gene (88) antibody
    • Myeloid differentiation primary response gene 88 antibody
    • Myeloid differentiation primary response gene antibody
    • Myeloid differentiation primary response protein MyD88 antibody
    • OTTHUMP00000161718 antibody
    • OTTHUMP00000208595 antibody
    • OTTHUMP00000209058 antibody
    • OTTHUMP00000209059 antibody
    • OTTHUMP00000209060 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MyD88 knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: MOLT4 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab107585 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.
    ab107585 was shown to recognize MyD88 when MyD88 knockout samples were used, along with additional cross-reactive bands. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab107585 and ab176560 (loading control to alpha tubulin) were diluted 5 µg/mL and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Intracellular flow cytometric analysis of MyD88 antibody (ab107585) in Jurkat cells (0.5 ug). Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-MyD88 antibody. Secondary antibody- anti-mouse IgG PE conjugated.
  • Immunocytochemical analysis of MyD88 antibody (ab107585) in MCF-7 cells using an isotype control (top) and Myd88 Antibody (5 µg/ml; bottom).
  • All lanes : Anti-MyD88 antibody [4D6] (ab107585) at 5 µg/ml

    Lane 1 : Human ovary cell lysate
    Lane 2 : Human prostate cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Jurkat cell lysate with Immunizing peptide

    Secondary
    All lanes : Goat anti-mouse Ig HRP

    Predicted band size: 33 kDa

References

This product has been referenced in:

  • Sun Y  et al. Exercise intervention alleviates nerve injury by the suppression of inflammatory mediator expression via the TLR4/NF-?B signaling pathway. Exp Ther Med 16:2922-2930 (2018). Read more (PubMed: 30233666) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (B-cells)
Loading amount
30 µg
Specification
B-cells
Treatment
BL19LP overexpress MyD88
Gel Running Conditions
Reduced Denaturing (10% SDS-Acrylamide gel 150V, 1h)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 08 2013

Question
Answer

DISCOUNT CODE: ABTRIAL-0000
Ablaufdatum: 01 März. 2012

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab107585 in Maus zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer ABTRIAL-0000, deckt den Wert von ab107585 ab und kann zum Kauf weiterer Produkte eingesetzt werden.

Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in Maus eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unseren Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

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