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  1. Link

    myd88-antibody-ab2064.pdf

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Signal Transduction Adapters Cytoplasmic
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Anti-MyD88 antibody (ab2064)

  • Datasheet
Reviews (10)Q&A (13)References (121)

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Western blot - Anti-MyD88 antibody (ab2064)
  • Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
  • Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
  • Western blot - Anti-MyD88 antibody (ab2064)

Key features and details

  • Rabbit polyclonal to MyD88
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-MyD88 antibody [EPR21824] (ab219413)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-MyD88 antibody
    See all MyD88 primary antibodies
  • Description

    Rabbit polyclonal to MyD88
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MyD88 aa 279-296 (C terminal).
    Sequence:

    CTKSWFWTRLAKALSLP


    (Peptide available as ab7878)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HEK293, HT29, K562, HepG2, A549 and Jurkat whole cell lysate (ab7899). ICC/IF: Jurkat and K562 cells.
  • General notes

    MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human MyD88 protein (ab114217)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (9)
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).
ICC/IF
Use a concentration of 20 µg/ml.
Notes
WB
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).
ICC/IF
Use a concentration of 20 µg/ml.

Target

  • Function

    Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
  • Sequence similarities

    Contains 1 death domain.
    Contains 1 TIR domain.
  • Domain

    The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q99836 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4615 Human
    • Omim: 602170 Human
    • SwissProt: Q99836 Human
    • Unigene: 82116 Human
    • Alternative names

      • Mutant myeloid differentiation primary response 88 antibody
      • MYD 88 antibody
      • Myd88 antibody
      • MYD88_HUMAN antibody
      • MYD88D antibody
      • Myeloid differentiation marker 88 antibody
      • Myeloid differentiation primary response 88 antibody
      • Myeloid differentiation primary response gene (88) antibody
      • Myeloid differentiation primary response gene 88 antibody
      • Myeloid differentiation primary response gene antibody
      • Myeloid differentiation primary response protein MyD88 antibody
      • OTTHUMP00000161718 antibody
      • OTTHUMP00000208595 antibody
      • OTTHUMP00000209058 antibody
      • OTTHUMP00000209059 antibody
      • OTTHUMP00000209060 antibody
      see all

    Images

    • Western blot - Anti-MyD88 antibody (ab2064)
      Western blot - Anti-MyD88 antibody (ab2064)
      All lanes : Anti-MyD88 antibody (ab2064) at 2 µg/ml

      Lane 1 : HEK293 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : HT29 cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

      Predicted band size: 33 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
      Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
      Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed Jurkat cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

       

    • Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
      Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
      Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed K562 cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

       

    • Western blot - Anti-MyD88 antibody (ab2064)
      Western blot - Anti-MyD88 antibody (ab2064)This image is courtesy of an anonymous Abreview
      All lanes : Anti-MyD88 antibody (ab2064) at 1 µg/ml

      Lanes 1-2 : Wild type MEFs whole cell lysate
      Lanes 3-4 : MyD88 knockout MEFs whole cell lysate

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 33 kDa
      Observed band size: 33 kDa


      Exposure time: 15 seconds

      See Abreview

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (121)

    Publishing research using ab2064? Please let us know so that we can cite the reference in this datasheet.

    ab2064 has been referenced in 121 publications.

    • Åsberg SE  et al. Frontline Science: Antibiotic treatment routes Mycobacterium avium to phagolysosomes without triggering proinflammatory cytokine production in human M?s. J Leukoc Biol 109:23-33 (2021). PubMed: 32531827
    • Wu L  et al. Silencing TLR4/MyD88/NF-?B Signaling Pathway Alleviated Inflammation of Corneal Epithelial Cells Infected by ISE. Inflammation 44:633-644 (2021). PubMed: 33174138
    • Su W  et al. Suppression of TLR4-MyD88 signaling pathway attenuated chronic mechanical pain in a rat model of endometriosis. J Neuroinflammation 18:65 (2021). PubMed: 33673857
    • Zhang X  et al. miR-139-5p protects septic mice with acute lung injury by inhibiting Toll-like receptor 4/Myeloid differentiation factor 88/Nuclear factor-&mac_kgr;B signaling pathway. Clinics (Sao Paulo) 76:e2484 (2021). PubMed: 33681946
    • Huang SQ  et al. Abdominal paracentesis drainage attenuates intestinal inflammation in rats with severe acute pancreatitis by inhibiting the HMGB1-mediated TLR4 signaling pathway. World J Gastroenterol 27:815-834 (2021). PubMed: 33727772
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 23 Abreviews or Q&A

    Western blot abreview for Anti-MyD88 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Mouse Cell lysate - whole cell (MEFs)
    Specification
    MEFs
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 02 2015

    Western blot abreview for Anti-MyD88 antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (macrophages cell line)
    Loading amount
    50 µg
    Specification
    macrophages cell line
    Treatment
    LPS
    Gel Running Conditions
    Reduced Denaturing (10-12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Varkha Agrawal

    Verified customer

    Submitted Sep 10 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MyD88 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human Normal Tonsil)
    Specification
    Human Normal Tonsil
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: DAKO Flex High pH-PTLink
    Permeabilization
    No
    Blocking step
    (agent) for 5 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 27 2011

    Western blot abreview for Anti-MyD88 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (liver)
    Loading amount
    20 µg
    Specification
    liver
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 17 2010

    Western blot abreview for Anti-MyD88 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (smooth muscle cells)
    Loading amount
    15 µg
    Specification
    smooth muscle cells
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 21 2009

    Question

    Thanks. It is great if you can send me a hard copy of these inserts..   Regards Shally  : 10/27/11  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 27 2011

    Answer

    Thank you for your reply. I will be happy to mail the datasheets to you. Are you having trouble accessing the datasheets online? I only ask so that I can inform our IT department if there are problems. Could you also please send me the address that you would like the datasheets sent to. Thank you and I look forward to your reply.

    Read More

    Abcam Scientific Support

    Answered on Oct 27 2011

    Question

    Dear Technical, would you please mail us hard copy of thee inserts as we have confusion reading these files.  We hope to be able to receive them tomorrow if you send us today.  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 27 2011

    Answer

    The datasheets are accessible from the product pages on the abcam.com website. You could try and print them from there. If you still have difficulty, please let me know and I will mail the datasheets to you.

    Read More

    Abcam Scientific Support

    Answered on Oct 27 2011

    Question

    Dear Technical, we do not have the product insert for the following items. would you please send us copy asap?   Best regard

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2011

    Answer

    Thank you for contacting Abcam. Please find attached the missing datasheets. If there is anything else I can help you with, please let me kn

    Read More

    Abcam Scientific Support

    Answered on Oct 25 2011

    Question

    I received the vial, but there does not appear to be anything in it.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 11 2011

    Answer

    Thank you for contacting us. I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial which you will receive tomorrow. Please do not hesitate to contact us if you need anything further.

    Read More

    Abcam Scientific Support

    Answered on Oct 11 2011

    Question

    Thank you for your email.I understand that GR 4851-3 & GR 4851-4 antibodies are from the same production batch, but then, I cannot understand why the two aliquots of antibodies gave different specificity results. I attached two pictures from Western blots done with liver samples from mice (20 ug).  Conditions are exactly the same,  in fact the first aliquot of antibody GR 4851-3, was used to establish those conditions in our lab (see below).  However, when we used a second aliquot of the same antibody (GR 4851-4) with the same liver samples that were used before, the results were quite disappointing. We observed many unspecific binging of the primary antibody, which did not diminished with higher dilutions (from 1:250 to 1:500) of the antibody or with longer blocking time (from 1 hour to 2hours) with BSA 2.5% . Western Blot Condition established with the GR 4851-3 aliquot:·       Blocking in BSA 2.5% or 5% in TTBS 1% for 1 hour or 2 hours (Blocking in Milk was also tried)·       Washing 3-4 times for 5 min in TTBS 1%·       1° antibody: different dilution factors have been tested and the one that gave better results was the 1:250 in 10 ml BSA 2.5%. Overnight incubation.·       2° antibody in BSA 2.5% TTBS 1% (unspecific binding given by the 2° antibody alone were not detected) Now that we received another GR 4851-4 aliquot, what are we supposed to do?If we test this new aliquot with the same conditions and with the same samples and have not good results, are we then entitled to be refunded? If this new aliquot works well, will we be refunded for the one that did not give good results? How will we know if further aliquots will be good or not? Considering the high amount of antibody which is required for each WB procedure (about 40 ul) and the total amount of antibody contained in each aliquot 100ul, you understand that we do not have the chance to waste antibody to test if the aliquot is a good one or not. Looking forward to hear from you soon.  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 06 2011

    Answer

    Thank you for your reply. I am sorry that you did not have a good experience with a previous vial of ab2064 lot GR 4851-4. I would like to reassure you that if you use this new antibody and do not obtain the desired results, I would be happy to replace this antibody free of charge. I encourage you to test this new vial of ab2064. However, please let me know if the new vial works for you. Also, can you provide an order number for the previous vial of ab2064 you purchased that did not work? I wish you best of luck with your experiments. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Abcam Scientific Support

    Answered on Oct 06 2011

    1-10 of 23 Abreviews or Q&A

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