Anti-MyD88 antibody (ab2064)

Rabbit polyclonal MyD88 antibody. Validated in WB and tested in Mouse, Rat, Human. Cited in 46 publication(s). Independently reviewed in 10 review(s). Immunogen corresponding to synthetic peptide.

Reviews (10)Q&A (13)References (63)

Overview

  • Product name

    Anti-MyD88 antibody
    See all MyD88 primary antibodies

  • Description

    Rabbit polyclonal to MyD88

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human MyD88 aa 279-296 (C terminal).
    Sequence:

    CTKSWFWTRLAKALSLP


    (Peptide available as ab7878)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Jurkat whole cell lysate (ab7899).

  • General notes

    MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab2064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).

Target

  • Function

    Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.

  • Tissue specificity

    Ubiquitous.

  • Involvement in disease

    Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.

  • Sequence similarities

    Contains 1 death domain.
    Contains 1 TIR domain.

  • Domain

    The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Mutant myeloid differentiation primary response 88 antibody
    • MYD 88 antibody
    • Myd88 antibody
    • MYD88_HUMAN antibody
    • MYD88D antibody
    • Myeloid differentiation marker 88 antibody
    • Myeloid differentiation primary response 88 antibody
    • Myeloid differentiation primary response gene (88) antibody
    • Myeloid differentiation primary response gene 88 antibody
    • Myeloid differentiation primary response gene antibody
    • Myeloid differentiation primary response protein MyD88 antibody
    • OTTHUMP00000161718 antibody
    • OTTHUMP00000208595 antibody
    • OTTHUMP00000209058 antibody
    • OTTHUMP00000209059 antibody
    • OTTHUMP00000209060 antibody
    see all

Images

  • Western blot - Anti-MyD88 antibody (ab2064)
    Western blot - Anti-MyD88 antibody (ab2064)This image is courtesy of an anonymous Abreview
    All lanes : Anti-MyD88 antibody (ab2064) at 1 µg/ml

    Lanes 1-2 : Wild type MEFs whole cell lysate
    Lanes 3-4 : MyD88 knockout MEFs whole cell lysate

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 15 seconds

    See Abreview

  • Western blot - Anti-MyD88 antibody (ab2064)
    Western blot - Anti-MyD88 antibody (ab2064)
    Anti-MyD88 antibody (ab2064) at 1/500 dilution + Jurkat Whole Cell Lysate

    Predicted band size: 33 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • DE Moura CFG  et al. Evaluation of the Chemopreventive Activity of Grape Skin Extract Using Medium-term Oral Carcinogenesis Assay Induced by 4-Nitroquinoline 1-Oxide. Anticancer Res 39:177-182 (2019). Read more (PubMed: 30591456) »
  • Cai J  et al. Recombinant fimbriae protein of Porphyromonas gingivalis induces an inflammatory response via the TLR4/NF-?B signaling pathway in human peripheral blood mononuclear cells. Int J Mol Med 43:1430-1440 (2019). Read more (PubMed: 30664173) »
See all 63 Publications for this product

Publishing research using ab2064? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 23 Abreviews or Q&A

Western blot abreview for Anti-MyD88 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Cell lysate - whole cell (MEFs)
Specification
MEFs
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Feb 02 2015

Western blot abreview for Anti-MyD88 antibody

 Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophages cell line)
Loading amount
50 µg
Specification
macrophages cell line
Treatment
LPS
Gel Running Conditions
Reduced Denaturing (10-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Varkha Agrawal

Verified customer

Submitted Sep 10 2012

Question

Thanks. It is great if you can send me a hard copy of these inserts..   Regards Shally  : 10/27/11  

Read More
Answer

Thank you for your reply. I will be happy to mail the datasheets to you. Are you having trouble accessing the datasheets online? I only ask so that I can inform our IT department if there are problems. Could you also please send me the address that you would like the datasheets sent to. Thank you and I look forward to your reply.

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Question

Dear Technical, would you please mail us hard copy of thee inserts as we have confusion reading these files.  We hope to be able to receive them tomorrow if you send us today.  

Read More
Answer

The datasheets are accessible from the product pages on the abcam.com website. You could try and print them from there. If you still have difficulty, please let me know and I will mail the datasheets to you.

Read More

Question

Dear Technical, we do not have the product insert for the following items. would you please send us copy asap?   Best regard

Read More
Answer

Thank you for contacting Abcam. Please find attached the missing datasheets. If there is anything else I can help you with, please let me kn

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Question

I received the vial, but there does not appear to be anything in it.

Read More
Answer

Thank you for contacting us. I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial which you will receive tomorrow. Please do not hesitate to contact us if you need anything further.

Read More

Question

Thank you for your email.I understand that GR 4851-3 & GR 4851-4 antibodies are from the same production batch, but then, I cannot understand why the two aliquots of antibodies gave different specificity results. I attached two pictures from Western blots done with liver samples from mice (20 ug).  Conditions are exactly the same,  in fact the first aliquot of antibody GR 4851-3, was used to establish those conditions in our lab (see below).  However, when we used a second aliquot of the same antibody (GR 4851-4) with the same liver samples that were used before, the results were quite disappointing. We observed many unspecific binging of the primary antibody, which did not diminished with higher dilutions (from 1:250 to 1:500) of the antibody or with longer blocking time (from 1 hour to 2hours) with BSA 2.5% . Western Blot Condition established with the GR 4851-3 aliquot:·       Blocking in BSA 2.5% or 5% in TTBS 1% for 1 hour or 2 hours (Blocking in Milk was also tried)·       Washing 3-4 times for 5 min in TTBS 1%·       1° antibody: different dilution factors have been tested and the one that gave better results was the 1:250 in 10 ml BSA 2.5%. Overnight incubation.·       2° antibody in BSA 2.5% TTBS 1% (unspecific binding given by the 2° antibody alone were not detected) Now that we received another GR 4851-4 aliquot, what are we supposed to do?If we test this new aliquot with the same conditions and with the same samples and have not good results, are we then entitled to be refunded? If this new aliquot works well, will we be refunded for the one that did not give good results? How will we know if further aliquots will be good or not? Considering the high amount of antibody which is required for each WB procedure (about 40 ul) and the total amount of antibody contained in each aliquot 100ul, you understand that we do not have the chance to waste antibody to test if the aliquot is a good one or not. Looking forward to hear from you soon.  

Read More
Answer

Thank you for your reply. I am sorry that you did not have a good experience with a previous vial of ab2064 lot GR 4851-4. I would like to reassure you that if you use this new antibody and do not obtain the desired results, I would be happy to replace this antibody free of charge. I encourage you to test this new vial of ab2064. However, please let me know if the new vial works for you. Also, can you provide an order number for the previous vial of ab2064 you purchased that did not work? I wish you best of luck with your experiments. Please do not hesitate to contact us if you have any additional questions.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MyD88 antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Normal Tonsil)
Specification
Human Normal Tonsil
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: DAKO Flex High pH-PTLink
Permeabilization
No
Blocking step
(agent) for 5 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted May 27 2011

Western blot abreview for Anti-MyD88 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
20 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Aug 17 2010

Western blot abreview for Anti-MyD88 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (smooth muscle cells)
Loading amount
15 µg
Specification
smooth muscle cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Apr 21 2009

1-10 of 23 Abreviews or Q&A

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