Anti-MyD88 antibody (ab2064)
Key features and details
- Rabbit polyclonal to MyD88
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MyD88 antibody
See all MyD88 primary antibodies -
Description
Rabbit polyclonal to MyD88 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MyD88 aa 250-350 (C terminal).
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Positive control
- WB: HEK293, HT29, K562, HepG2, A549 and Jurkat whole cell lysate (ab7899). ICC/IF: HeLa, Jurkat and K562 cells.
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General notes
MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (9) |
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).
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ICC/IF |
Use a concentration of 20 µg/ml.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa). |
ICC/IF
Use a concentration of 20 µg/ml. |
Target
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Function
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age. -
Sequence similarities
Contains 1 death domain.
Contains 1 TIR domain. -
Domain
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4615 Human
- Omim: 602170 Human
- SwissProt: Q99836 Human
- Unigene: 82116 Human
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Alternative names
- Mutant myeloid differentiation primary response 88 antibody
- MYD 88 antibody
- Myd88 antibody
see all
Images
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Immunofluorescent analysis of methanol-fixed HeLa cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green).
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All lanes : Anti-MyD88 antibody (ab2064) at 2 µg/ml
Lane 1 : HeLa WT cell lysate
Lane 2 : MyD88 KO HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 33 kDa1 h incubation at RT in 5% NFDM/TBST.
beta-actin was used as a loading control at 1 µg/mL.
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All lanes : Anti-MyD88 antibody (ab2064) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : MYD88 knockout A549 cell lysate
Lane 3 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Western blot: Anti-MYD88 antibody (ab2064) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab2064 was shown to bind specifically to MYD88. A band was observed at 35 kDa in wild-type A549 cell lysates with no signal observed at this size in MYD88 knockout cell line ab286715 (knockout cell lysate ab290793). To generate this image, wild-type and MYD88 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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All lanes : Anti-MyD88 antibody (ab2064) at 2 µg/ml
Lane 1 : A549 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HT29 cell lysate
Lane 5 : HEK293 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 33 kDa -
Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed Jurkat cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed K562 cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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All lanes : Anti-MyD88 antibody (ab2064) at 1 µg/ml
Lanes 1-2 : Wild type MEFs whole cell lysate
Lanes 3-4 : MyD88 knockout MEFs whole cell lysate
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 15 seconds
Datasheets and documents
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Datasheet download
References (137)
ab2064 has been referenced in 137 publications.
- Guo S et al. Specific Silencing of Microglial Gene Expression in the Rat Brain by Nanoparticle-Based Small Interfering RNA Delivery. ACS Appl Mater Interfaces 14:5066-5079 (2022). PubMed: 35041392
- Krishnan M et al. Molecular mechanism underlying the TLR4 antagonistic and antiseptic activities of papiliocin, an insect innate immune response molecule. Proc Natl Acad Sci U S A 119:e2115669119 (2022). PubMed: 35238667
- Fei ZY et al. Analysis of bacterial spectrum, activin A, and CD64 in chronic obstructive pulmonary disease patients complicated with pulmonary infections. World J Clin Cases 10:2382-2392 (2022). PubMed: 35434072
- Yang J et al. Fuzi-Lizhong Decoction Alleviates Nonalcoholic Fatty Liver Disease by Blocking TLR4/MyD88/TRAF6 Signaling. Evid Based Complement Alternat Med 2022:1637701 (2022). PubMed: 36065267
- Zhang Y et al. Triptolide in the treatment of systemic lupus erythematosus - regulatory effects on miR-146a in B cell TLR7 signaling pathway in mice. Front Pharmacol 13:952775 (2022). PubMed: 36210830