Overview

  • Product name
    Anti-MyD88 antibody
    See all MyD88 primary antibodies
  • Description
    Rabbit polyclonal to MyD88
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MyD88 aa 279-296 (C terminal).
    Sequence:

    CTKSWFWTRLAKALSLP


    (Peptide available as ab7878)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Jurkat whole cell lysate.
  • General notes
    MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab2064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).Can be blocked with Human MyD88 peptide (ab7878).

Target

  • Function
    Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
  • Sequence similarities
    Contains 1 death domain.
    Contains 1 TIR domain.
  • Domain
    The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Mutant myeloid differentiation primary response 88 antibody
    • MYD 88 antibody
    • Myd88 antibody
    • MYD88_HUMAN antibody
    • MYD88D antibody
    • Myeloid differentiation marker 88 antibody
    • Myeloid differentiation primary response 88 antibody
    • Myeloid differentiation primary response gene (88) antibody
    • Myeloid differentiation primary response gene 88 antibody
    • Myeloid differentiation primary response gene antibody
    • Myeloid differentiation primary response protein MyD88 antibody
    • OTTHUMP00000161718 antibody
    • OTTHUMP00000208595 antibody
    • OTTHUMP00000209058 antibody
    • OTTHUMP00000209059 antibody
    • OTTHUMP00000209060 antibody
    see all

Images

  • Western blot - Anti-MyD88 antibody (ab2064)
    Western blot - Anti-MyD88 antibody (ab2064)This image is courtesy of an anonymous Abreview
    All lanes : Anti-MyD88 antibody (ab2064) at 1 µg/ml

    Lanes 1-2 : Wild type MEFs whole cell lysate
    Lanes 3-4 : MyD88 knockout MEFs whole cell lysate

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 15 seconds

    See Abreview

  • Western blot - Anti-MyD88 antibody (ab2064)
    Western blot - Anti-MyD88 antibody (ab2064)
    Anti-MyD88 antibody (ab2064) at 1/500 dilution + Jurkat Whole Cell Lysate

    Predicted band size: 33 kDa
    Observed band size: 35 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Morale MG  et al. HPV-transformed cells exhibit altered HMGB1-TLR4/MyD88-SARM1 signaling axis. Sci Rep 8:3476 (2018). WB . Read more (PubMed: 29472602) »
  • Zhou W  et al. Galectin-3 activates TLR4/NF-?B signaling to promote lung adenocarcinoma cell proliferation through activating lncRNA-NEAT1 expression. BMC Cancer 18:580 (2018). Read more (PubMed: 29788922) »

See all 36 Publications for this product

Publishing research using ab2064? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

23 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-MyD88 antibody

 Excellent
Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Cell lysate - whole cell (MEFs)
Specification
MEFs
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Feb 02 2015

Western blot abreview for Anti-MyD88 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophages cell line)
Loading amount
50 µg
Specification
macrophages cell line
Treatment
LPS
Gel Running Conditions
Reduced Denaturing (10-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
Username

Dr. Varkha Agrawal

Verified customer

Submitted Sep 10 2012

Thanks. It is great if you can send me a hard copy of these inserts..   Regards Shally  : 10/27/11

Thank you for your reply. I will be happy to mail the datasheets to you. Are you having trouble accessing the datasheets online? I only ask so that I can inform our IT department if there are problems. Could you also please send me the address that you...

Read More

Dear Technical, would you please mail us hard copy of thee inserts as we have confusion reading these files.  We hope to be able to receive them tomorrow if you send us today.

The datasheets are accessible from the product pages on the abcam.com website. You could try and print them from there. If you still have difficulty, please let me know and I will mail the datasheets to you.

Dear Technical, we do not have the product insert for the following items. would you please send us copy asap?   Best regard

Thank you for contacting Abcam. Please find attached the missing datasheets. If there is anything else I can help you with, please let me kn

I received the vial, but there does not appear to be anything in it.

Thank you for contacting us. I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial which you will receive tomorrow. P...

Read More

Thank you for your email.I understand that GR 4851-3 & GR 4851-4 antibodies are from the same production batch, but then, I cannot understand why the two aliquots of antibodies gave different specificity results. I attached two pictures from Western bl...

Read More

Thank you for your reply. I am sorry that you did not have a good experience with a previous vial of ab2064 lot GR 4851-4. I would like to reassure you that if you use this new antibody and do not obtain the desired results, I would be happy to rep...

Read More

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MyD88 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Normal Tonsil)
Specification
Human Normal Tonsil
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: DAKO Flex High pH-PTLink
Permeabilization
No
Blocking step
(agent) for 5 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted May 27 2011

Western blot abreview for Anti-MyD88 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
20 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Aug 17 2010

Western blot abreview for Anti-MyD88 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (smooth muscle cells)
Loading amount
15 µg
Specification
smooth muscle cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Apr 21 2009

1-10 of 23 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up