Key features and details
- Goat polyclonal to MyD88
- Suitable for: IHC-P, WB, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MyD88 antibody
See all MyD88 primary antibodies
DescriptionGoat polyclonal to MyD88
Tested applicationsSuitable for: IHC-P, WB, Sandwich ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow, Dog
- Human T cell line MOLT4 cell lysate. Human thymus lysate. Human tonsil tissue sections.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab28763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
1 hour primary incubation is recommended for this product.
|Sandwich ELISA||Use a concentration of 5 - 10 µg/ml. Recombinant Antigen captured by rabbit antibody and ab28763 used as reporter|
FunctionAdapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
Involvement in diseaseDefects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
Sequence similaritiesContains 1 death domain.
Contains 1 TIR domain.
DomainThe intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
- Information by UniProt
- Mutant myeloid differentiation primary response 88 antibody
- MYD 88 antibody
- Myd88 antibody
Anti-MyD88 antibody (ab28763) at 0.2 µg/ml + Human thymus lysate at 35 µg
Predicted band size: 33 kDa
Observed band size: 33 kDa
Primary antibody incubated for 1 hour and detected using chemiluminescence.
Anti-MyD88 antibody (ab28763) at 0.01 µg/ml + Human T cell line MOLT4 cell lysate, 30µg total protein per lane
Predicted band size: 33 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Primary incubation was for 1 hour.
Detected using chemiluminescence.
ab28763 (4µg/ml) staining of paraffin embedded Human Tonsil shows staining of clusters of cells some of which are likely plasma cells. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
ab28763 has been referenced in 3 publications.
- Yang C et al. Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway. Stem Cells Int 2020:2134565 (2020). PubMed: 32300366
- Liu D et al. MyD88 expression in the rat dental follicle: implications for osteoclastogenesis and tooth eruption. Eur J Oral Sci 118:333-41 (2010). PubMed: 20662905
- Szajnik M et al. TLR4 signaling induced by lipopolysaccharide or paclitaxel regulates tumor survival and chemoresistance in ovarian cancer. Oncogene 28:4353-63 (2009). IHC-P ; Human . PubMed: 19826413