Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MyD88 antibody [EPR21824] (ab219413)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21824] to MyD88
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MyD88 antibody [EPR21824]
    See all MyD88 primary antibodies

  • Description

    Rabbit monoclonal [EPR21824] to MyD88

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Mouse MyD88 aa 50-150. The exact sequence is proprietary.
    Database link: P22366

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate. HepG2 and Ramos whole cell lysate. Rat and mouse liver lysate. RAW 264.7 and A20 whole cell lysate. Mouse lung lysate. IP: RAW 264.7 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab219413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/1000. Predicted molecular weight: 33 kDa.

Target

  • Function

    Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.

  • Tissue specificity

    Ubiquitous.

  • Involvement in disease

    Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.

  • Sequence similarities

    Contains 1 death domain.
    Contains 1 TIR domain.

  • Domain

    The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Mutant myeloid differentiation primary response 88 antibody
    • MYD 88 antibody
    • Myd88 antibody
    • MYD88_HUMAN antibody
    • MYD88D antibody
    • Myeloid differentiation marker 88 antibody
    • Myeloid differentiation primary response 88 antibody
    • Myeloid differentiation primary response gene (88) antibody
    • Myeloid differentiation primary response gene 88 antibody
    • Myeloid differentiation primary response gene antibody
    • Myeloid differentiation primary response protein MyD88 antibody
    • OTTHUMP00000161718 antibody
    • OTTHUMP00000208595 antibody
    • OTTHUMP00000209058 antibody
    • OTTHUMP00000209059 antibody
    • OTTHUMP00000209060 antibody
    see all

Images

  • Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)
    Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)
    All lanes : Anti-MyD88 antibody [EPR21824] (ab219413) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : MyD88 knockout HAP1 whole cell lysate
    Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 4 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit monoclonal [EPR21824] to MyD88 (ab219413) at 1/100000 dilution

    Predicted band size: 33 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    ab219413 was shown to specifically react with MyD88 in wild-type HAP1 cells as signal was lost in MyD88 knockout cells. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab219413 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique

  • Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)
    Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)
    All lanes : Anti-MyD88 antibody [EPR21824] (ab219413) at 1/1000 dilution

    Lane 1 : Rat liver lysate
    Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 3 : A20 (Mouse reticulum sarcoma B lymphocyte) cell lysate
    Lane 4 : Mouse liver lysate
    Lane 5 : Mouse lung lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (ab219413)
    Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (ab219413)

    MyD88 was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab219413 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219413 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.


    Lane 1: RAW 264.7 whole cell lysate 10 µg (Input).
    Lane 2: ab219413 IP in RAW 264.7 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219413 in RAW 264.7 whole cell lysate (-).
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab219413? Please let us know so that we can cite the reference in this datasheet.

ab219413 has been referenced in 1 publication.

  • Rong Y  et al. Structural characterization of an active polysaccharide of longan and evaluation of immunological activity. Carbohydr Polym 213:247-256 (2019). PubMed: 30879666

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