Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-MyD88 antibody [EPR21824] (ab219413)

Submit a review Submit a question References (1)

Overview

  • Product name

    Anti-MyD88 antibody [EPR21824]
    See all MyD88 primary antibodies

  • Description

    Rabbit monoclonal [EPR21824] to MyD88

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Mouse MyD88 aa 50-150. The exact sequence is proprietary.
    Database link: P22366

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate. HepG2 and Ramos whole cell lysate. Rat and mouse liver lysate. RAW 264.7 and A20 whole cell lysate. Mouse lung lysate. IP: RAW 264.7 whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab219413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/1000. Predicted molecular weight: 33 kDa.

Target

  • Function

    Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.

  • Tissue specificity

    Ubiquitous.

  • Involvement in disease

    Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.

  • Sequence similarities

    Contains 1 death domain.
    Contains 1 TIR domain.

  • Domain

    The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Mutant myeloid differentiation primary response 88 antibody
    • MYD 88 antibody
    • Myd88 antibody
    • MYD88_HUMAN antibody
    • MYD88D antibody
    • Myeloid differentiation marker 88 antibody
    • Myeloid differentiation primary response 88 antibody
    • Myeloid differentiation primary response gene (88) antibody
    • Myeloid differentiation primary response gene 88 antibody
    • Myeloid differentiation primary response gene antibody
    • Myeloid differentiation primary response protein MyD88 antibody
    • OTTHUMP00000161718 antibody
    • OTTHUMP00000208595 antibody
    • OTTHUMP00000209058 antibody
    • OTTHUMP00000209059 antibody
    • OTTHUMP00000209060 antibody
    see all

Images

  • Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)
    Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)
    All lanes : Anti-MyD88 antibody [EPR21824] (ab219413) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : MyD88 knockout HAP1 whole cell lysate
    Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 4 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit monoclonal [EPR21824] to MyD88 (ab219413) at 1/100000 dilution

    Predicted band size: 33 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    ab219413 was shown to specifically react with MyD88 in wild-type HAP1 cells as signal was lost in MyD88 knockout cells. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab219413 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique

  • Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)
    Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)
    All lanes : Anti-MyD88 antibody [EPR21824] (ab219413) at 1/1000 dilution

    Lane 1 : Rat liver lysate
    Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 3 : A20 (Mouse reticulum sarcoma B lymphocyte) cell lysate
    Lane 4 : Mouse liver lysate
    Lane 5 : Mouse lung lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (ab219413)
    Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (ab219413)

    MyD88 was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab219413 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219413 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.


    Lane 1: RAW 264.7 whole cell lysate 10 µg (Input).
    Lane 2: ab219413 IP in RAW 264.7 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219413 in RAW 264.7 whole cell lysate (-).
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

References

This product has been referenced in:

  • Rong Y  et al. Structural characterization of an active polysaccharide of longan and evaluation of immunological activity. Carbohydr Polym 213:247-256 (2019). Read more (PubMed: 30879666) »
See 1 Publication for this product

Publishing research using ab219413? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab219413.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up