Recombinant Anti-MyD88 antibody [EPR21824] (ab219413)
![Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)](https://www.abcam.com/ps/products/219/ab219413/Images/ab219413-313603-anti-myd88-antibody-epr21824-western-blot.jpg "Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21824] to MyD88
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MyD88 antibody [EPR21824]
See all MyD88 primary antibodies -
Description
Rabbit monoclonal [EPR21824] to MyD88 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1 whole cell lysate. HepG2 and Ramos whole cell lysate. Rat and mouse liver lysate. RAW 264.7 and A20 whole cell lysate. Mouse lung lysate. IP: RAW 264.7 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21824 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30.
|
|
| WB |
1/1000. Predicted molecular weight: 33 kDa.
|
| Notes |
|---|
|
IP
1/30. |
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WB
1/1000. Predicted molecular weight: 33 kDa. |
Target
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Function
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age. -
Sequence similarities
Contains 1 death domain.
Contains 1 TIR domain. -
Domain
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4615 Human
- Entrez Gene: 17874 Mouse
- Entrez Gene: 301059 Rat
- Omim: 602170 Human
- SwissProt: Q99836 Human
- SwissProt: P22366 Mouse
- SwissProt: Q6Y1S1 Rat
- Unigene: 82116 Human
see all -
Alternative names
- Mutant myeloid differentiation primary response 88 antibody
- MYD 88 antibody
- Myd88 antibody
see all
Images
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Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)All lanes : Anti-MyD88 antibody [EPR21824] (ab219413) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MyD88 knockout HAP1 whole cell lysate
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 4 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit monoclonal [EPR21824] to MyD88 (ab219413) at 1/100000 dilution
Predicted band size: 33 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
ab219413 was shown to specifically react with MyD88 in wild-type HAP1 cells as signal was lost in MyD88 knockout cells. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. ab219413 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique
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![Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)](https://www.abcam.com/ps/products/219/ab219413/Images/ab219413-313604-anti-myd88-antibody-epr21824-western-blot.jpg)
Western blot - Anti-MyD88 antibody [EPR21824] (ab219413)All lanes : Anti-MyD88 antibody [EPR21824] (ab219413) at 1/1000 dilution
Lane 1 : Rat liver lysate
Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : A20 (Mouse reticulum sarcoma B lymphocyte) cell lysate
Lane 4 : Mouse liver lysate
Lane 5 : Mouse lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 33 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST
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![Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (ab219413)](https://www.abcam.com/ps/products/219/ab219413/Images/ab219413-313607-anti-myd88-antibody-epr21824-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (ab219413)MyD88 was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab219413 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219413 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: RAW 264.7 whole cell lysate 10 µg (Input).
Lane 2: ab219413 IP in RAW 264.7 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219413 in RAW 264.7 whole cell lysate (-).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes. -
![Anti-MyD88 antibody [EPR21824] (ab219413)](https://www.abcam.com/ps/products/219/ab219413/Images/ab219413-4-benefits-of-recombinant-antibodies.png)
Anti-MyD88 antibody [EPR21824] (ab219413)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (5)
ab219413 has been referenced in 5 publications.
- Chen G et al. Sepsis-induced acute lung injury in young rats is relieved by calycosin through inactivating the HMGB1/MyD88/NF-?B pathway and NLRP3 inflammasome. Int Immunopharmacol 96:107623 (2021). PubMed: 33857805
- Tong L et al. Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota. Theranostics 11:8570-8586 (2021). PubMed: 34373759
- Wang T et al. LEF1 mediates osteoarthritis progression through circRNF121/miR-665/MYD88 axis via NF-?B signaling pathway. Cell Death Dis 11:598 (2020). PubMed: 32732957
- Ye Y et al. Aloperine suppresses LPS-induced macrophage activation through inhibiting the TLR4/NF-?B pathway. Inflamm Res 69:375-383 (2020). PubMed: 32144444
- Rong Y et al. Structural characterization of an active polysaccharide of longan and evaluation of immunological activity. Carbohydr Polym 213:247-256 (2019). PubMed: 30879666
