Key features and details
- Mouse monoclonal [OTI1B4] to MyD88
- Suitable for: WB, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-MyD88 antibody [OTI1B4]
See all MyD88 primary antibodies
DescriptionMouse monoclonal [OTI1B4] to MyD88
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human MyD88.
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY MyD88 cDNA; Jerkat, and MOLT4 cell lysates. Flow Cyt: HEK293T cells transfected with pCMV6-ENTRY MyD88 overexpression plasmid; HeLa and Jurkat cells. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY MyD88; HeLa and Jurkat cells.
The clone number has been updated from 1B4 to OTI1B4, both clone numbers name the same clone.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography
Our Abpromise guarantee covers the use of ab119048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 33 kDa.|
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionAdapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
Involvement in diseaseDefects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
Sequence similaritiesContains 1 death domain.
Contains 1 TIR domain.
DomainThe intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
- Information by UniProt
- Mutant myeloid differentiation primary response 88 antibody
- MYD 88 antibody
- Myd88 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MyD88 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: Molt-4 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab119048 observed at 37 kDa. Red - loading control, ab176560, observed at 52 kDa.
ab119048 was shown to specifically react with MyD88 when MyD88 knockout samples were used. Wild-type and MyD88 knockout samples were subjected to SDS-PAGE. Ab119048 and ab176560 (loading control to alpha Tubulin) were diluted at 1/2000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 andGoat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-MyD88 antibody [OTI1B4] (ab119048) at 1/2000 dilution
Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY MyD88 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 33 kDa
ab119048 at 1/100 dilution staining MyD88 in COS7 cells transiently transfected with pCMV6-ENTRY MyD88 by Immunofluorescence.
ab119048 at 1/100 dilution, staining MyD88 in HEK293T cells transfected with pCMV6-ENTRY MyD88 overexpress plasmid(Red), or an empty vector control plasmid (Blue) by Flow Cytometry.
ab119048 at 1/100 dilution staining MyD88 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
ab119048 at 1/100 dilution staining MyD88 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
ab119048 has been referenced in 1 publication.
- Xue X et al. Immunoregulatory Role of MicroRNA-21 in Macrophages in Response to Bacillus Calmette-Guerin Infection Involves Modulation of the TLR4/MyD88 Signaling Pathway. Cell Physiol Biochem 42:91-102 (2017). PubMed: 28494464