Product nameMyD88 overexpression 293T lysate (whole cell)
See all MyD88 lysates
DescriptionMyD88 overexpression lysate
ab94066 is a 293T cell transfected lysate in which Human MyD88 has been transiently over-expressed using a pCMV-MyD88 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Tissue specificity: Ubiquitous. Disease: Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age. Similarity: Contains 1 death domain. Contains 1 TIR domain. Domain: The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
Our Abpromise guarantee covers the use of ab94066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94066 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-MyD88 antibody (ab89576) at 1/500 dilution
Lane 1 :
MyD88 overexpression 293T lysate (whole cell) (ab94066) at 25 µg
Lane 2 : 293T non-transfected lysate
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94066 has not yet been referenced specifically in any publications.