Overview

  • Product name
    Anti-Myelin Basic Protein antibody [12]
    See all Myelin Basic Protein primary antibodies
  • Description
    Rat monoclonal [12] to Myelin Basic Protein
  • Host species
    Rat
  • Specificity
    The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Mapped by Geyson method to DENPW method.
  • Tested applications
    Suitable for: IHC-FoFr, IHC-P, WB, ELISA, RIA, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog, Human, Pig, Apteronotus leptorhynchus
  • Immunogen

    Full length protein (Cow).

  • Epitope
    Amino acids 82-87 (DENPVV).

Properties

Applications

Our Abpromise guarantee covers the use of ab7349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 18678887
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 23584610

Target

  • Function
    The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • Tissue specificity
    MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • Involvement in disease
    Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • Sequence similarities
    Belongs to the myelin basic protein family.
  • Developmental stage
    Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • Post-translational
    modifications
    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • Cellular localization
    Myelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • Database links
  • Alternative names
    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Images

  • ab7349 staining Myelin Basic Protein in human trigeminal ganglia tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/1000 in 10% NDS) for 16 hours at 22°C. ab150165, a goat anti-rat IgG H&L (Alexa Fluor® 488) preadsorbed polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Anti-Myelin Basic Protein antibody [12] (ab7349) + Mouse brain tissue lysate

    Observed band size: 19,26 kDa
    why is the actual band size different from the predicted?

  • ab7349 staining Myelin Basic Protein in murine cerebral cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was not fixed. Samples were blocked with 2.5% serum for 1 hour followed by incubation with the primary antibody at a 1/200 dilution for 18 hours. A Biotin-conjugated rabbit anti-rat polyclonal was used as the secondary antibody at a 1/500 dilution.
  • ab7349 at 1/100 staining mouse spinal cord tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 3% serum prior to incubation with the antibody for 18 hours. A biotinylated horse anti-rat antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Eisemann T  et al. An advanced glioma cell invasion assay based on organotypic brain slice cultures. BMC Cancer 18:103 (2018). Read more (PubMed: 29378533) »
  • Weider M  et al. Nfat/calcineurin signaling promotes oligodendrocyte differentiation and myelination by transcription factor network tuning. Nat Commun 9:899 (2018). Read more (PubMed: 29500351) »
See all 97 Publications for this product

Customer reviews and Q&As

1-10 of 32 Abreviews or Q&A

Answer

Thank you for your enquiry. I do not have the accession number for the immunogen; however, the amino acid sequence to which the antibody binds is DENPVV. I have updated the datasheet for future reference. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA ph 9
Permeabilization
No
Specification
Brain tissue
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Formaldehyde

Herr Dr. Markus Kipp

Verified customer

Submitted Sep 07 2018

Application
IHC - Wholemount
Sample
Mouse Tissue (Brain, striatum)
Specification
Brain, striatum

Abcam user community

Verified customer

Submitted May 23 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Trigeminal Ganglia)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6.0
Permeabilization
No
Specification
Trigeminal Ganglia
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

April Rempel

Verified customer

Submitted May 13 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: Rm°C
Sample
Human Tissue sections (Human Myelin/Oligodendrocytes in Mouse Shiverer Br)
Specification
Human Myelin/Oligodendrocytes in Mouse Shiverer Br
Permeabilization
Yes - Triton
Fixative
Paraformaldehyde

Dr. Fraser Sim

Verified customer

Submitted Sep 16 2014

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Zebra finch Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Mr. Adam Piraino

Verified customer

Submitted Mar 28 2013

Answer

Thank you for your reply.

The following antibodies are suitable for your primary antibody ab7349 which is a rat IgG2a:

Biotinylated:

ab99668

https://www.abcam.com/index.html?datasheet=99668 (or use the following: https://www.abcam.com/index.html?datasheet=99668).

HRP conjugated:

ab99667

https://www.abcam.com/index.html?datasheet=99667 (or use the following: https://www.abcam.com/index.html?datasheet=99667).

ab106783

https://www.abcam.com/index.html?datasheet=106783 (or use the following: https://www.abcam.com/index.html?datasheet=106783).

Fluorochrome conjugated:

ab99669

https://www.abcam.com/index.html?datasheet=99669 (or use the following: https://www.abcam.com/index.html?datasheet=99669).

ab99665

https://www.abcam.com/index.html?datasheet=99665 (or use the following: https://www.abcam.com/index.html?datasheet=99665).

Extensive Protocols can be found here:

https://www.abcam.com/index.html?pageconfig=popular_protocols

https://www.abcam.com/index.html?pageconfig=resource&rid=11383

or listen to one of our webinars:

https://www.abcam.com/index.html?pageconfig=resource&rid=15175

I hope this information is helpful and wish you a good weekend.

Read More

Answer

Before I can recommend a secondary antibody to you, I would need to know the following:

What application are you using, IHC-P or IHC-Fr?

Shall the antibody be conjugated with an enzyme or with a Fluorochrom?

Will you use the primary antibody in co-immunostaining experiments with other primary antibodies?

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 23 2013

Answer

Thank you for your enquiry.

I can confirm that the anti-Myelin Basic Protein antibody [12] - Oligodendrocyte Marker ab7349 is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml. I would suggest starting your experiment with a dilution of 1/1000 and optimise further to achieve the best results. A checkerboard titration of the dilution of the antibody could be performed.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

1-10 of 32 Abreviews or Q&A

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