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Neuroscience Cell Adhesion Proteins Membrane Proteins
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Anti-Myelin Basic Protein antibody [22] (ab11223)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [22] (ab11223)

    Key features and details

    • Mouse monoclonal [22] to Myelin Basic Protein
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG2b

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    Overview

    • Product name

      Anti-Myelin Basic Protein antibody [22]
      See all Myelin Basic Protein primary antibodies
    • Description

      Mouse monoclonal [22] to Myelin Basic Protein
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Full length protein (Human).

    • Epitope

      ab11223 recognises an epitope of myelin basic protein between amino acids 84-89.
    • General notes

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituent: Tissue culture supernatant
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      22
    • Myeloma

      Sp2/0
    • Isotype

      IgG2b
    • Research areas

      • Neuroscience
      • Cell Adhesion Proteins
      • Membrane Proteins
      • Neuroscience
      • Cell Type Marker
      • Glia marker
      • Oligodendrocyte marker
      • Tags & Cell Markers
      • Cell Type Markers
      • Neuroscience Markers
      • Glial

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
    • Recombinant Protein

      • Recombinant Human Myelin Basic Protein (Tagged) (ab43614)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab11223 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    Application Abreviews Notes
    IHC-P
    Use at an assay dependent concentration.
    Notes
    IHC-P
    Use at an assay dependent concentration.

    Target

    • Function

      The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
    • Tissue specificity

      MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
    • Involvement in disease

      Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
    • Sequence similarities

      Belongs to the myelin basic protein family.
    • Developmental stage

      Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
    • Post-translational
      modifications

      Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
      The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
      Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
    • Cellular localization

      Myelin membrane. Cytoplasmic side of myelin.
    • Target information above from: UniProt accession P02686 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4155 Human
      • Omim: 159430 Human
      • SwissProt: P02686 Human
      • Unigene: 551713 Human
      • Alternative names

        • GDB antibody
        • Golli MBP antibody
        • Golli MBP; myelin basic protein antibody
        • Hemopoietic MBP antibody
        • HMBPR antibody
        • HUGO antibody
        • MBP antibody
        • MBP_CAVPO antibody
        • MBP_HUMAN antibody
        • MGC99675 antibody
        • MLD antibody
        • Myelin A1 protein antibody
        • Myelin A1 Protein, basic antibody
        • Myelin basic protein antibody
        • Myelin Deficient antibody
        • Myelin membrane encephalitogenic protein antibody
        • OTTHUMP00000163776 antibody
        • OTTHUMP00000174387 antibody
        • OTTHUMP00000174388 antibody
        • SHI antibody
        • Shiverer antibody
        • SP antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [22] (ab11223)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [22] (ab11223)
        ab11223 staining Myelin Basic Protein of white matter in human brain section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab11223? Please let us know so that we can cite the reference in this datasheet.

      ab11223 has been referenced in 1 publication.

      • Cai S  et al. Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann Cells. Stem Cell Reports 9:1097-1108 (2017). IHC ; Human . PubMed: 28890164

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