Key features and details
- Chicken polyclonal to Myelin Basic Protein
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgY
Product nameAnti-Myelin Basic Protein antibody
See all Myelin Basic Protein primary antibodies
DescriptionChicken polyclonal to Myelin Basic Protein
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
Mixture of 3 Human Myelin Basic Protein synthetic peptides, representing the different forms of the protein (alternate transcription from a single gene).
- ICC/IF: Rat mixed neuron/glial cultures WB: Rat spinal cord whole tissue homogenate, or peripheral nerve homogenate
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.066% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein L purified
Our Abpromise guarantee covers the use of ab123499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Predicted molecular weight: 33 kDa. A prominent band is often shown at about 20 kDa. This is a convergence of 3 different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa.|
FunctionThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
Tissue specificityMBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Involvement in diseaseNote=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
Sequence similaritiesBelongs to the myelin basic protein family.
Developmental stageExpression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
modificationsSeveral charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
Cellular localizationMyelin membrane. Cytoplasmic side of myelin.
- Information by UniProt
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
ab123499, at a 1/1000 dilution, staining Myelin Basic Protein in an oligodendrocyte from Rat mixed neuron/glial culture by Immunofluorescence.
Anti-Myelin Basic Protein antibody (ab123499) at 1/10000 dilution + whole tissue homogenate of Rat spinal cord
Predicted band size: 33 kDa
Acrylamide gel concentration: 12%
ab123499 has been referenced in 1 publication.
- Orefice NS et al. miRNA profile is altered in a modified EAE mouse model of multiple sclerosis featuring cortical lesions. Elife 9:N/A (2020). PubMed: 32940597