Key features and details
- Rabbit polyclonal to Myelin Basic Protein
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-Myelin Basic Protein antibody
See all Myelin Basic Protein primary antibodies
DescriptionRabbit polyclonal to Myelin Basic Protein
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Guinea pig
- WB: Mouse brain lysate. IHC-P: Rat brain tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/1000. Predicted molecular weight: 33 kDa.|
FunctionThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
Tissue specificityMBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Involvement in diseaseNote=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
Sequence similaritiesBelongs to the myelin basic protein family.
Developmental stageExpression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
modificationsSeveral charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
Cellular localizationMyelin membrane. Cytoplasmic side of myelin.
- Information by UniProt
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue, labeling Myelin Basic Protein using ab216590 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Anti-Myelin Basic Protein antibody (ab216590) at 1/300 dilution + mouse brain lysate
Developed using the ECL technique.
Predicted band size: 33 kDa
Immunofluorescence analysis of formalin-fixed rat brain tissue, labeling Myelin Basic Protein using ab216590 at a 1/200 dilution; followed by secondary antibody PE-conjugated goat anti-rabbit lgG at a 1/200 dilution. Cell nuclei were stained with 5 µL/mL DAPI (blue).
Immunofluorescence analysis of formalin-fixed rat brain tissue, labeling Myelin Basic Protein using ab216590 at a 1/200 dilution; followed by secondary antibody PE-conjugated goat anti-rabbit lgG at a 1/200 dilution.
ab216590 has not yet been referenced specifically in any publications.