For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    myelin-basic-protein-antibody-epr6652-ab133620.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Cell Adhesion Proteins Membrane Proteins
Share by email
RecombinantRabMAb

Recombinant Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)

  • Datasheet
Reviews (4) Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
  • Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
  • Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
  • Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6652] to Myelin Basic Protein
  • Suitable for: WB, IHC-P
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
Product image
PE Anti-Iba1 antibody [EPR6136(2)] (ab209942)
Peptide
Mouse Myelin Basic Protein peptide (ab40389)

View more associated products

Overview

  • Product name

    Anti-Myelin Basic Protein antibody [EPR6652]
    See all Myelin Basic Protein primary antibodies
  • Description

    Rabbit monoclonal [EPR6652] to Myelin Basic Protein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Myelin Basic Protein aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P02686-1

  • Positive control

    • Human fetal brain lysate, Myelin Basic Protein recombinant protein, Human oligodendroglioma and Human brain tissues
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6652
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial

Associated products

  • Alternative Versions

    • Anti-Myelin Basic Protein antibody [EPR6652] - BSA and Azide free (ab218542)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Myelin Basic Protein (Tagged) (ab43614)

Applications

Our Abpromise guarantee covers the use of ab133620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
    • Tissue specificity

      MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
    • Involvement in disease

      Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
    • Sequence similarities

      Belongs to the myelin basic protein family.
    • Developmental stage

      Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
    • Post-translational
      modifications

      Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
      The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
      Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
    • Cellular localization

      Myelin membrane. Cytoplasmic side of myelin.
    • Target information above from: UniProt accession P02686 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4155 Human
      • Omim: 159430 Human
      • SwissProt: P02686 Human
      • Unigene: 551713 Human
      • Alternative names

        • GDB antibody
        • Golli MBP antibody
        • Golli MBP; myelin basic protein antibody
        • Hemopoietic MBP antibody
        • HMBPR antibody
        • HUGO antibody
        • MBP antibody
        • MBP_CAVPO antibody
        • MBP_HUMAN antibody
        • MGC99675 antibody
        • MLD antibody
        • Myelin A1 protein antibody
        • Myelin A1 Protein, basic antibody
        • Myelin basic protein antibody
        • Myelin Deficient antibody
        • Myelin membrane encephalitogenic protein antibody
        • OTTHUMP00000163776 antibody
        • OTTHUMP00000174387 antibody
        • OTTHUMP00000174388 antibody
        • SHI antibody
        • Shiverer antibody
        • SP antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)

        Immunohistochemical analysis of paraffin embedded Human brain tissue labelling Myelin Basic Protein with ab133620 antibody at a dilution of 1/250.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)

        ab133620 showing positive staining in Astrocytoma tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Anti-Myelin Basic Protein antibody [EPR6652] (ab133620) at 1/10000 dilution + Human fetal brain lysate at 10 µg

        Secondary
        HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 33 kDa
        Observed band size: 19 kDa
        why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)

        Immunohistochemical analysis of paraffin embedded Human oligodendroglioma tissue labelling Myelin Basic Protein with ab133620 antibody at a dilution of 1/250.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Anti-Myelin Basic Protein antibody [EPR6652] (ab133620) at 1/10000 dilution + Myelin Basic Protein recombinant protein at 0.01 µg

        Predicted band size: 33 kDa



        Note: the molecular weight of the recombinant protein is 46 kDa.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)

        ab133620 showing negative staining in Skeletal muscle tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)

        ab133620 showing negative staining in Normal kidney tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab133620? Please let us know so that we can cite the reference in this datasheet.

      ab133620 has been referenced in 2 publications.

      • Sharma AD  et al. Engineering a 3D functional human peripheral nerve in vitro using the Nerve-on-a-Chip platform. Sci Rep 9:8921 (2019). PubMed: 31222141
      • Naudí A  et al. Region-specific vulnerability to lipid peroxidation and evidence of neuronal mechanisms for polyunsaturated fatty acid biosynthesis in the healthy adult human central nervous system. Biochim Biophys Acta 1862:485-495 (2017). WB ; Human . PubMed: 28185952

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-Myelin Basic Protein antibody [EPR6652]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Rat Tissue sections (Adult sciatic nerve)
      Permeabilization
      Yes - 0.3% Tx-100
      Specification
      Adult sciatic nerve
      Blocking step
      5% NGS, 0.4% BSA, 0.1% TX-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Wesley Anderson

      Verified customer

      Submitted Aug 05 2020

      Immunohistochemistry (Frozen sections) abreview for Anti-Myelin Basic Protein antibody [EPR6652]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Adult sciatic nerve)
      Permeabilization
      Yes - 0.3% Tx-100
      Specification
      Adult sciatic nerve
      Blocking step
      5% NGS, 0.4% BSA, 0.1% TX-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Wesley Anderson

      Verified customer

      Submitted Aug 05 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myelin Basic Protein antibody [EPR6652]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 04 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myelin Basic Protein antibody [EPR6652]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Nerve)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
      Permeabilization
      No
      Specification
      Nerve
      Blocking step
      PB ab64226 as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 15 2017

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.