Recombinant Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-147500-anti-myelin-basic-protein-antibody-epr6652-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6652] to Myelin Basic Protein
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Myelin Basic Protein antibody [EPR6652]
See all Myelin Basic Protein primary antibodies -
Description
Rabbit monoclonal [EPR6652] to Myelin Basic Protein -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Myelin Basic Protein aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: P02686-1 -
Positive control
- Human fetal brain lysate, Myelin Basic Protein recombinant protein, Human oligodendroglioma and Human brain tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6652 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 33 kDa). | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. -
Tissue specificity
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. -
Involvement in disease
Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). -
Sequence similarities
Belongs to the myelin basic protein family. -
Developmental stage
Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter. -
Post-translational
modificationsSeveral charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. -
Cellular localization
Myelin membrane. Cytoplasmic side of myelin. - Information by UniProt
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Database links
- Entrez Gene: 4155 Human
- Omim: 159430 Human
- SwissProt: P02686 Human
- Unigene: 551713 Human
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Alternative names
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
Immunohistochemical analysis of paraffin embedded Human brain tissue labelling Myelin Basic Protein with ab133620 antibody at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-147497-anti-myelin-basic-protein-antibody-epr6652-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)ab133620 showing positive staining in Astrocytoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-147502-anti-myelin-basic-protein-antibody-epr6652-western-blot.jpg)
Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)Anti-Myelin Basic Protein antibody [EPR6652] (ab133620) at 1/10000 dilution + Human fetal brain lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-147499-anti-myelin-basic-protein-antibody-epr6652-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)Immunohistochemical analysis of paraffin embedded Human oligodendroglioma tissue labelling Myelin Basic Protein with ab133620 antibody at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-147501-anti-myelin-basic-protein-antibody-epr6652-western-blot.jpg)
Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)Anti-Myelin Basic Protein antibody [EPR6652] (ab133620) at 1/10000 dilution + Myelin Basic Protein recombinant protein at 0.01 µg
Predicted band size: 33 kDa
Note: the molecular weight of the recombinant protein is 46 kDa. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-147498-anti-myelin-basic-protein-antibody-epr6652-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)ab133620 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-147496-anti-myelin-basic-protein-antibody-epr6652-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)ab133620 showing negative staining in Normal kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)](https://www.abcam.com/ps/products/133/ab133620/Images/ab133620-8-benefits-of-recombinant-antibodies.png)
Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
Protocols
Datasheets and documents
References (2)
ab133620 has been referenced in 2 publications.
- Sharma AD et al. Engineering a 3D functional human peripheral nerve in vitro using the Nerve-on-a-Chip platform. Sci Rep 9:8921 (2019). PubMed: 31222141
- Naudí A et al. Region-specific vulnerability to lipid peroxidation and evidence of neuronal mechanisms for polyunsaturated fatty acid biosynthesis in the healthy adult human central nervous system. Biochim Biophys Acta 1862:485-495 (2017). WB ; Human . PubMed: 28185952
