Recombinant
RabMAb

Recombinant Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)

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Overview

  • Product name

    Anti-Myelin Basic Protein antibody [EPR6652]
    See all Myelin Basic Protein primary antibodies

  • Description

    Rabbit monoclonal [EPR6652] to Myelin Basic Protein

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Myelin Basic Protein aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P02686-1

  • Positive control

    • Human fetal brain lysate, Myelin Basic Protein recombinant protein, Human oligodendroglioma and Human brain tissues

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

    • Tissue specificity

      MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

    • Involvement in disease

      Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).

    • Sequence similarities

      Belongs to the myelin basic protein family.

    • Developmental stage

      Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.

    • Post-translational
      modifications

      Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
      The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
      Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.

    • Cellular localization

      Myelin membrane. Cytoplasmic side of myelin.
    • Information by UniProt

    • Database links

      • Alternative names

        • GDB antibody
        • Golli MBP antibody
        • Golli MBP; myelin basic protein antibody
        • Hemopoietic MBP antibody
        • HMBPR antibody
        • HUGO antibody
        • MBP antibody
        • MBP_CAVPO antibody
        • MBP_HUMAN antibody
        • MGC99675 antibody
        • MLD antibody
        • Myelin A1 protein antibody
        • Myelin A1 Protein, basic antibody
        • Myelin basic protein antibody
        • Myelin Deficient antibody
        • Myelin membrane encephalitogenic protein antibody
        • OTTHUMP00000163776 antibody
        • OTTHUMP00000174387 antibody
        • OTTHUMP00000174388 antibody
        • SHI antibody
        • Shiverer antibody
        • SP antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)

        Immunohistochemical analysis of paraffin embedded Human brain tissue labelling Myelin Basic Protein with ab133620 antibody at a dilution of 1/250.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)

        ab133620 showing positive staining in Astrocytoma tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Anti-Myelin Basic Protein antibody [EPR6652] (ab133620) at 1/10000 dilution + Human fetal brain lysate at 10 µg

        Secondary
        HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 33 kDa
        Observed band size: 19 kDa
        why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)

        Immunohistochemical analysis of paraffin embedded Human oligodendroglioma tissue labelling Myelin Basic Protein with ab133620 antibody at a dilution of 1/250.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Anti-Myelin Basic Protein antibody [EPR6652] (ab133620) at 1/10000 dilution + Myelin Basic Protein recombinant protein at 0.01 µg

        Predicted band size: 33 kDa



        Note: the molecular weight of the recombinant protein is 46 kDa.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)

        ab133620 showing negative staining in Skeletal muscle tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)

        ab133620 showing negative staining in Normal kidney tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • Sharma AD  et al. Engineering a 3D functional human peripheral nerve in vitro using the Nerve-on-a-Chip platform. Sci Rep 9:8921 (2019). Read more (PubMed: 31222141) »
      • Naudí A  et al. Region-specific vulnerability to lipid peroxidation and evidence of neuronal mechanisms for polyunsaturated fatty acid biosynthesis in the healthy adult human central nervous system. Biochim Biophys Acta 1862:485-495 (2017). WB ; Human . Read more (PubMed: 28185952) »
      See all 2 Publications for this product

      Publishing research using ab133620? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myelin Basic Protein antibody [EPR6652]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 04 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myelin Basic Protein antibody [EPR6652]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Nerve)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
      Permeabilization
      No
      Specification
      Nerve
      Blocking step
      PB ab64226 as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 15 2017

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