Recombinant
RabMAb

Recombinant Anti-Myelin Basic Protein antibody [EPR6652] - BSA and Azide free (ab218542)

Overview

  • Product name

    Anti-Myelin Basic Protein antibody [EPR6652] - BSA and Azide free
    See all Myelin Basic Protein primary antibodies
  • Description

    Rabbit monoclonal [EPR6652] to Myelin Basic Protein - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Myelin Basic Protein aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P02686-1

  • Positive control

    • IHC-P: Human brain tissue.
  • General notes

    Ab218542 is the carrier-free version of ab133620. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab218542 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab218542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 33 kDa).

Target

  • Function

    The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • Tissue specificity

    MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • Involvement in disease

    Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • Sequence similarities

    Belongs to the myelin basic protein family.
  • Developmental stage

    Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • Post-translational
    modifications

    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • Cellular localization

    Myelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • Database links

  • Alternative names

    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human oligodendroglioma tissue labelling Myelin Basic Protein with ab133620 antibody at a dilution of 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133620).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • ab133620 showing negative staining in Skeletal muscle tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133620).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • ab133620 showing positive staining in Astrocytoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133620).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • ab133620 showing negative staining in Normal kidney tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133620).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded human brain tissue labeling Myelin Basic Protein with ab133620 antibody at a dilution of 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133620).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

References

ab218542 has not yet been referenced specifically in any publications.

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