Overview

  • Product name
    Anti-Myelin Basic Protein antibody (HRP)
    See all Myelin Basic Protein primary antibodies
  • Description
    Rabbit polyclonal to Myelin Basic Protein (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WB, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig
  • Immunogen

    Full length bovine Myelin Basic Protein

  • General notes

    The purified antibody is conjugated to peroxidase (HRP) through reductive amination. Reductive amination with Ab/HRP ratio: 1/20.

Properties

Applications

Our Abpromise guarantee covers the use of ab77895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
IHC-P Use at an assay dependent dilution.
Sandwich ELISA 1/10000. To be used as detection antibody. Pair with ab28541 - Rabbit polyclonal to Myelin Basic Protein

Target

  • Function
    The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • Tissue specificity
    MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • Involvement in disease
    Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • Sequence similarities
    Belongs to the myelin basic protein family.
  • Developmental stage
    Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • Post-translational
    modifications
    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • Cellular localization
    Myelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • Database links
  • Alternative names
    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Images

  • Sandwich ELISA detection of mouse Myelin Basic Protein in the plasma with un-optimized sensitivity 0.1 ng/mL.
  • Fixed Immobilized Anti-MBP (ab28541): 2 ug/mL, 100 ul/well
    Captured Antigen: Swine MBP , range from 1 to 90 ng/mL
    Detection: anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt
  • Immobilized Anti-MBP (ab28541): range from 0.125 to 2 µg/ml, 100 µl/well
    Fixed Captured Antigen: Swine MBP, 500 ng/mL
    Detection: anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt

References

This product has been referenced in:
  • Zahednasab H  et al. The protective effect of rifampicin on behavioral deficits, biochemical, and neuropathological changes in a cuprizone model of demyelination. Cytokine 113:417-426 (2019). Read more (PubMed: 30539784) »
  • Kuzina E  et al. Glatiramer Acetate and Nanny Proteins Restrict Access of the Multiple Sclerosis Autoantigen Myelin Basic Protein to the 26S Proteasome. Biomed Res Int 2014:926394 (2014). WB ; Cow . Read more (PubMed: 25276831) »
See all 3 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Answer

Thank you for contacting Abcam.

On Friday we discussed your use of ab77895 in IHC-P and lack of a positive signal. I recommended some protocol tips, specifically attempting alternative antigen retrieval methods and increasing your primary antibody concentration. Antigen retrieval specifics:
1. Attempt 95oC, 30 minute antigen retrieval incubation of slides in a coplin jar in the water bath with Citrate buffer.
2. Attempt 37oC, 30 minute enzymatic antigen retrieval incubation in a coplin jar in the water bath using proteinase K or pepsin antigen retrieval.

Specific formulations for proteinase K and pepsin antigen retrieval buffers can be found through the following link:
http://www.ihcworld.com/epitope_retrieval.htm

Further IHC-P troubleshooting steps can be found through the link below:
https://www.abcam.com/index.html?pageconfig=resource&rid=11384

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for your reply.

Unforrtunately we do not have any references for IHC with this product, only WB. Generally for a purified antibody, a dilution of 5 ug/ml is a good starting point for the primary antibody. The lab recommends 1 hr at RT, but you can also try overnight at 4C to see if that gives you a better signal.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Question
Answer

Thank you very much for your call yesterday and for your patience while I have been in touch with the lab.

During IHC testing of this antibody, the lab incubated the primary antibody with the tissue for 1 hour at room temperature.

I hope this will be useful, but if you have further questions or need anything else, please let me know and I'll be happy to help.

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Question
Answer

Thank you very much for your reply.

I'm sending a free of charge vial of ab65988, which will ship out tomorrow and arrive on Monday. The reference number for this shipment is ***.

Please keep me updated about the results with this antibody, and let me know if there is anything else that we can do for you.

Read More

Answer

Thank you again for contacting us and letting us know about this issue.

I'm wondering if you have received my last email?

Ab40390 has only been tested with mouse and rat samples, so we can not guarantee that it will work with human samples. If you would like a rabbit polyclonal antibody, we have ab65988 which has been tested in Western blot with human samples-

https://www.abcam.com/Myelin-Basic-Protein-antibody-Carboxyterminal-end-ab65988.html

Would you like this antibody instead? We do have this in stock so we can ship it out tonight and it will arrive on Monday. Please let me know and I will arrange this promptly.

Read More

Answer

Thank you very much for your reply.

Ab40390 has only been tested with mouse and rat samples, so we can not guarantee that it will work with human samples. If you would like a rabbit polyclonal antibody, we have ab65988 which has been tested in Western blot with human samples-

https://www.abcam.com/Myelin-Basic-Protein-antibody-Carboxyterminal-end-ab65988.html

Would you like this antibody instead? We do have this in stock sowe can ship it out tonight and it will arrive tomorrow. Please let me know and I will arrange this promptly.

Read More

Answer

Thank you very much for your reply and for sending the additional information.

Would you prefer a replacement with a different MBP antibody? We have several that react with human samples, such as ab7349, which has been cited in 19publications and has received a 4-star rating from other users-

https://www.abcam.com/Myelin-Basic-Protein-antibody-12-Oligodendrocyte-Marker-ab7349.html

I can also issue a credit or refund if you prefer. Please let me know which you prefer, and I will process it promptly.

Have a nice day.

Read More

Answer

Thank you very much for your reply and for sending the additional information.

We do fully guarantee all of our products to work as stated on the datasheet for up to 6 months after purchase, so I can issue a replacement or refund in the case that an antibody fails to do so.

Could you please provide some information about the protocol used, for our records?

1) What species was the recombinantMBP sequence taken from?
2) How much protein was loaded per lane of the gel during the Western blot?
3) What kind of blocking solution was used?
4) What dilution of primary antibody was used, and how long was the antibody incubated on the membrane?
5) Are the secondary antibody and the detection reagents working with other primary antibodies?

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

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Question
Answer

Thank you for your call last week and for letting us know about the trouble with ab77895. My colleague who you spoke to on Friday is out of the office today, but I will be happy to help with this issue.

I believe my colleague was going to send you a testing discount code to submit an Abreview of ab77895 and receive a free primary antibody in exchange. Could you tell me what species of plant your samples were from? Could you also confirmyour original order number?I will need this information in order to issue the discount code.

I look forward to hearing from you. Please let me know if you have further questions or if there is anything else that we can do for you, and I'll be happy to help.

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