HRP Anti-Myelin Basic Protein antibody (ab77895)
Key features and details
- HRP Rabbit polyclonal to Myelin Basic Protein
- Suitable for: Sandwich ELISA
- Reacts with: Mouse, Human, Pig
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Myelin Basic Protein antibody
See all Myelin Basic Protein primary antibodies -
Description
HRP Rabbit polyclonal to Myelin Basic Protein -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Mouse, Human, Pig -
Immunogen
Full length native protein (purified) corresponding to Cow Myelin Basic Protein.
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General notes
The purified antibody is conjugated to peroxidase (HRP) through reductive amination. Reductive amination with Ab/HRP ratio: 1/20.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.00
Constituents: 5% BSA, 2.94% Sodium citrate, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ELISA pair antibody
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Recombinant Protein
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
1/10000. To be used as detection antibody. Pair with ab28541 - Rabbit polyclonal to Myelin Basic Protein
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Notes |
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Sandwich ELISA
1/10000. To be used as detection antibody. Pair with ab28541 - Rabbit polyclonal to Myelin Basic Protein |
Target
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Function
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. -
Tissue specificity
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. -
Involvement in disease
Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). -
Sequence similarities
Belongs to the myelin basic protein family. -
Developmental stage
Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter. -
Post-translational
modificationsSeveral charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. -
Cellular localization
Myelin membrane. Cytoplasmic side of myelin. - Information by UniProt
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Database links
- Entrez Gene: 4155 Human
- Entrez Gene: 17196 Mouse
- Omim: 159430 Human
- SwissProt: P02686 Human
- SwissProt: P04370 Mouse
- Unigene: 551713 Human
- Unigene: 252063 Mouse
- Unigene: 392350 Mouse
see all -
Alternative names
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
see all
Images
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Sandwich ELISA detection of mouse Myelin Basic Protein in the plasma with un-optimized sensitivity 0.1 ng/mL.
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Fixed Immobilized Anti-MBP (ab28541): 2 ug/mL, 100 ul/well
Captured Antigen: Swine MBP , range from 1 to 90 ng/mL
Detection: anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt -
Immobilized Anti-MBP (ab28541): range from 0.125 to 2 µg/ml, 100 µl/well
Fixed Captured Antigen: Swine MBP, 500 ng/mL
Detection: anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab77895 has been referenced in 4 publications.
- Zahednasab H et al. The protective effect of rifampicin on behavioral deficits, biochemical, and neuropathological changes in a cuprizone model of demyelination. Cytokine 113:417-426 (2019). PubMed: 30539784
- Humphreys CA et al. A protocol for precise comparisons of small vessel disease lesions between ex vivo magnetic resonance imaging and histopathology. Int J Stroke 14:310-320 (2019). PubMed: 30196792
- Kuzina E et al. Glatiramer Acetate and Nanny Proteins Restrict Access of the Multiple Sclerosis Autoantigen Myelin Basic Protein to the 26S Proteasome. Biomed Res Int 2014:926394 (2014). WB ; Cow . PubMed: 25276831
- Ioan-Facsinay A et al. Anti-cyclic citrullinated peptide antibodies are a collection of anti-citrullinated protein antibodies and contain overlapping and non-overlapping reactivities. Ann Rheum Dis 70:188-93 (2011). WB ; Human . PubMed: 20736390