Key features and details
- Mouse monoclonal [MBP101] to Myelin Basic Protein
- Suitable for: Flow Cyt
- Reacts with: Mouse, Rat, Sheep, Rabbit, Goat, Human
- Isotype: IgG2b
Product nameAnti-Myelin Basic Protein antibody [MBP101]
See all Myelin Basic Protein primary antibodies
DescriptionMouse monoclonal [MBP101] to Myelin Basic Protein
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Goat, Human
Predicted to work with: Non human primates
Purified human myelin basic protein.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Concentration information loading...
PurityProtein G purified
Purification notesPurified antibody
Our Abpromise guarantee covers the use of ab62631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
Tissue specificityMBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Involvement in diseaseNote=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
Sequence similaritiesBelongs to the myelin basic protein family.
Developmental stageExpression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
modificationsSeveral charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
Cellular localizationMyelin membrane. Cytoplasmic side of myelin.
- Information by UniProt
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
Overlay histogram showing SH-SY5Y cells stained with ab62631 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62631, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab62631 has been referenced in 56 publications.
- Buniatian GH et al. Antifibrotic Effects of Amyloid-Beta and Its Loss in Cirrhotic Liver. Cells 9:N/A (2020). PubMed: 32089540
- Cavanaugh M et al. RGD-Modified Nanofibers Enhance Outcomes in Rats after Sciatic Nerve Injury. J Funct Biomater 10:N/A (2019). PubMed: 31146396
- Yip PK et al. Docosahexaenoic acid reduces microglia phagocytic activity via miR-124 and induces neuroprotection in rodent models of spinal cord contusion injury. Hum Mol Genet 28:2427-2448 (2019). PubMed: 30972415
- Marinelli S et al. Innovative mouse model mimicking human-like features of spinal cord injury: efficacy of Docosahexaenoic acid on acute and chronic phases. Sci Rep 9:8883 (2019). PubMed: 31222077
- Sharma R et al. Chronic Exposure to High Altitude: Synaptic, Astroglial and Memory Changes. Sci Rep 9:16406 (2019). PubMed: 31712561