Overview

  • Product name

    Anti-Myelin Basic Protein antibody [MBP101]
    See all Myelin Basic Protein primary antibodies
  • Description

    Mouse monoclonal [MBP101] to Myelin Basic Protein
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, IHC-FoFr, ICC/IF, Flow Cyt, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Rabbit, Goat, Human
    Predicted to work with: Non human primates
  • Immunogen

    Purified human myelin basic protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab62631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 23700462
IHC-FoFr Use at an assay dependent concentration. PubMed: 24062649
ICC/IF 1/500.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • Tissue specificity

    MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • Involvement in disease

    Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • Sequence similarities

    Belongs to the myelin basic protein family.
  • Developmental stage

    Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • Post-translational
    modifications

    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • Cellular localization

    Myelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt
  • Database links

  • Alternative names

    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Images

  • Myelin destruction in the CNS

    Detection of myelin by immunohistochemistry for MBP in spinal cords of (A) untreated EAE mice, (B) HSV-Zeo treated EAE mice and (C) HSV-LIF treated EAE mice on day 21 post induction. The figure shows two sections of each group at two magnifications. The squares indicate the areas of inflammatory demyelinating infiltrates at higher magnification. Examples of inflammatory infiltrates disrupting the myelin are shown. Scale bars are shown in the figure.

    Myelin Basic Protein (MBP) was detected using ab62631 at 1/200 dilution in formalin fixed, paraffin-embedded mouse spinal cord tissue.

    (From Figure 5 of Nygardas et al)

  • ab62631 staining MBP in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with normal serum of species secondary antibody; antigen retrieval was by heat mediation in 10mM citric acid buffer. Samples were incubated with primary antibody (1/200) overnight at 4°C.

  • ICC/IF image of Rat Oligodendrocytes primary culture stained with ab62631. The cells on cover slip were incubated in 10% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab62631, 2µg/ml) overnight at +4°C. The secondary antibody was Alexa Fluor®568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab62631 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62631, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:

  • Thau-Zuchman O  et al. Brain Phospholipid Precursors Administered Post-Injury Reduce Tissue Damage and Improve Neurological Outcome in Experimental Traumatic Brain Injury. J Neurotrauma 36:25-42 (2019). Read more (PubMed: 29768974) »
  • Merolli A  et al. Reciprocal nerve staining (RNS) for the concurrent detection of choline acetyltransferase and myelin basic protein on paraffin-embedded sections. J Neurosci Methods 311:235-238 (2019). Read more (PubMed: 30391262) »
See all 52 Publications for this product

Customer reviews and Q&As

1-10 of 17 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (differentiated human iPSCs)
Specification
differentiated human iPSCs
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 17 2019

Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain)
Specification
Brain

Abcam user community

Verified customer

Submitted Aug 30 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (liver and brain)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Specification
liver and brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 17 2017

Application
Western blot
Sample
Rat Tissue lysate - whole (Liver and brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Liver and brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 17 2017

Application
Western blot
Sample
Rat Tissue lysate - whole (Sciatic nerve)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Sciatic nerve
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 22 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Sciatic nerve)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Sciatic nerve
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 22 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Free-floating brain sections, 25 micron)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, HCL
Permeabilization
Yes - 0.1% Triton-X100
Specification
Free-floating brain sections, 25 micron
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Ms. Erika Villanueva

Verified customer

Submitted Nov 18 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.3% Triton x-100 (in blocking step)
Fixative
Paraformaldehyde

Brandon Jun

Verified customer

Submitted Aug 13 2014

Application
Western blot
Sample
Mouse Tissue lysate - whole (Sciatic nerve)
Loading amount
30 µg
Specification
Sciatic nerve
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Dr. G.K Sakellariou

Verified customer

Submitted May 15 2013

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (intercostal nerve)
Specification
intercostal nerve
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 4% · Temperature: 25°C

Dr. Gillian Hunter

Verified customer

Submitted Feb 26 2013

1-10 of 17 Abreviews or Q&A

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