Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631)
![Immunocytochemistry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631)](https://www.abcam.com/ps/products/62/ab62631/Images/ab62631-5-anti-myelin-basic-protein-mbp101-mbp-immunocytochemistry-antibody-primary-neurons-div14-rat.jpg "Immunocytochemistry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631)")
Key features and details
- Mouse monoclonal [MBP101] to Myelin Basic Protein - BSA and Azide free
- Suitable for: ICC, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free
See all Myelin Basic Protein primary antibodies -
Description
Mouse monoclonal [MBP101] to Myelin Basic Protein - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: ICC, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Non human primates
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Immunogen
Purified human myelin basic protein.
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Positive control
- ICC: Primary hippocampal rat neurons/glia. Flow Cyt: SH-SY5Y cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified antibody -
Clonality
Monoclonal -
Clone number
MBP101 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use a concentration of 1 µg/ml.
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| Flow Cyt | (1) |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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ICC
Use a concentration of 1 µg/ml. |
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. -
Tissue specificity
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. -
Involvement in disease
Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). -
Sequence similarities
Belongs to the myelin basic protein family. -
Developmental stage
Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter. -
Post-translational
modificationsSeveral charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. -
Cellular localization
Myelin membrane. Cytoplasmic side of myelin. - Information by UniProt
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Database links
- Entrez Gene: 4155 Human
- Entrez Gene: 24547 Rat
- Omim: 159430 Human
- SwissProt: P02686 Human
- SwissProt: P02688 Rat
- Unigene: 551713 Human
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Alternative names
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
see all
Images
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Immunocytochemistry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631)
ab62631 staining Myelin Basic Protein in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab62631 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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![Flow Cytometry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631)](https://www.abcam.com/ps/products/62/ab62631/Images/ab62631-76041-anti-myelin-basic-protein-antibody-mbp101-flow-cytometry.jpg)
Flow Cytometry - Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free (ab62631)Overlay histogram showing SH-SY5Y cells stained with ab62631 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62631, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (82)
ab62631 has been referenced in 82 publications.
- Li R et al. Acidic fibroblast growth factor attenuates type 2 diabetes-induced demyelination via suppressing oxidative stress damage. Cell Death Dis 12:107 (2021). PubMed: 33479232
- Santos-Gil DF et al. Retinoid X receptor activation promotes re-myelination in a very old triple transgenic mouse model of Alzheimer's disease. Neurosci Lett 750:135764 (2021). PubMed: 33621639
- Brodovskaya A et al. Activation of the basal ganglia and indirect pathway neurons during frontal lobe seizures. Brain N/A:N/A (2021). PubMed: 33730155
- Liu H et al. SARM1 promotes neuroinflammation and inhibits neural regeneration after spinal cord injury through NF-?B signaling. Theranostics 11:4187-4206 (2021). PubMed: 33754056
- Aghajanian S et al. Facial nerve regeneration using silicone conduits filled with ammonia-functionalized graphene oxide and frankincense-embedded hydrogel. Inflamm Regen 41:13 (2021). PubMed: 33902759