Recombinant
RabMAb

Anti-Myelin expression factor 2 antibody [EPR10774(B)] (ab170946)

Overview

  • Product name
    Anti-Myelin expression factor 2 antibody [EPR10774(B)]
    See all Myelin expression factor 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR10774(B)] to Myelin expression factor 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Myelin expression factor 2. The exact sequence is proprietary.
    Database link: Q9P2K5

  • Positive control
    • Human fetal brain, Jurkat and MCF7 lysates; Human brain and testis tissues; MCF7 and Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, Glycerol
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10774(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 64 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    Myelin expression factor 2 (MEF2) is expressed early in the differentiation program and is suppressed by specific polypeptide growth factors. The ability of MEF2 to recognize conserved activating elements associated with multiple specific genes suggests that this factor may participate in the coordinate regulation of genes during myogenesis.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • MYEF2 antibody
    • KIAA1341 antibody
    • MEF 2 antibody
    • MST156 antibody
    • MYEF 2 antibody
    • Myelin expression factor 2 antibody
    see all

Images

  • Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate, labeling Myelin expression factor 2 using ab170946 at a 1/10 dilution.

  • All lanes : Anti-Myelin expression factor 2 antibody [EPR10774(B)] (ab170946) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 64 kDa

  • Immunohistochemistry analysis of paraffin-embedded Human testis tissue labeling Myelin expression factor 2 with ab170946 at 1/100 dilution.

  • Immunohistochemistry analysis of Human brain tissue labeling Myelin expression factor 2 with ab170946 at 1/100 dilution.

  • Immunofluorescence analysis of MCF7 cells labeling Myelin expression factor 2 with ab170946 at 1/100 dilution.

  • Flow cytometric analysis of permeabilized Jurkat cells labeling Myelin expression factor 2 with ab170946 at 1/10 in red and a rabbit IgG (negative control in green)

References

ab170946 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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