Recombinant
RabMAb

Recombinant Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335)

Overview

  • Product name

    Anti-Myelin expression factor 2 antibody [EPR10775(B)]
    See all Myelin expression factor 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR10775(B)] to Myelin expression factor 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Myelin expression factor 2 aa 550 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9P2K5

  • Positive control

    • SH-SY5Y, Jurkat, MCF-7, Raw 264.7, fetal brain and fetal liver lysates. Paraffin-embedded Human brain and Human testis tissue. SH-SY5Y cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10775(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 64 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance

      Myelin expression factor 2 (MEF2) is expressed early in the differentiation program and is suppressed by specific polypeptide growth factors. The ability of MEF2 to recognize conserved activating elements associated with multiple specific genes suggests that this factor may participate in the coordinate regulation of genes during myogenesis.
    • Cellular localization

      Nuclear
    • Database links

    • Alternative names

      • MYEF2 antibody
      • KIAA1341 antibody
      • MEF 2 antibody
      • MST156 antibody
      • MYEF 2 antibody
      • Myelin expression factor 2 antibody
      see all

    Images

    • Immunofluorescent analysis of SH-SY5Y cells labeling Myelin expression factor 2 with ab176335 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Myelin expression factor 2 with ab176335 at 1/250 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Myelin expression factor 2 with ab176335 at 1/250 dilution.

    • All lanes : Anti-Myelin expression factor 2 antibody [EPR10775(B)] (ab176335) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : MCF7 lysate
      Lane 4 : fetal brain lysate
      Lane 5 : fetal liver lysate
      Lane 6 : Raw 264.7 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 64 kDa

    References

    ab176335 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab176335.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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